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DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps
BACKGROUND: It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). METHOD: Twenty pairs of CAP samples with a diameter ≥ 1...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547352/ https://www.ncbi.nlm.nih.gov/pubmed/33061956 http://dx.doi.org/10.1155/2020/1798729 |
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author | Meng, Xianmei Liu, Na Jia, Yanbin Gong, Kerui Zhang, Jingjie Zhang, Wei Shao, Guo Dang, Tong |
author_facet | Meng, Xianmei Liu, Na Jia, Yanbin Gong, Kerui Zhang, Jingjie Zhang, Wei Shao, Guo Dang, Tong |
author_sort | Meng, Xianmei |
collection | PubMed |
description | BACKGROUND: It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). METHOD: Twenty pairs of CAP samples with a diameter ≥ 10 mm and corresponding normal colorectal mucosa (NCM) tissues from patients were used in the present study. The expression levels and activity of DNA methyltransferases (DNMTs) were measured in the CAP tissues. The global methylation and the promoter methylation level of 3 kinds of tumour suppressor gene were detected. RESULTS: mRNA and protein levels of DNMT3B were found to be elevated in the CAP tissues compared with the control tissue. Additionally, the methylation of long interspersed nuclear elements-1 (LINE-1/L1) was decreased in the CAP tissue. Furthermore, methylation of the promoter of a tumour suppressor gene Ras association domain family 1A (RASSF1A) was increased in the CAP tissues, while the mRNA levels of RASSF1A were decreased. CONCLUSIONS: These results suggest that the overexpression of DNMT3B may contribute to a role in the genesis of CAPs through the hypomethylation of chromosomes in the whole cell and promoter hypermethylation of RASSF1A. |
format | Online Article Text |
id | pubmed-7547352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75473522020-10-13 DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps Meng, Xianmei Liu, Na Jia, Yanbin Gong, Kerui Zhang, Jingjie Zhang, Wei Shao, Guo Dang, Tong Gastroenterol Res Pract Research Article BACKGROUND: It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). METHOD: Twenty pairs of CAP samples with a diameter ≥ 10 mm and corresponding normal colorectal mucosa (NCM) tissues from patients were used in the present study. The expression levels and activity of DNA methyltransferases (DNMTs) were measured in the CAP tissues. The global methylation and the promoter methylation level of 3 kinds of tumour suppressor gene were detected. RESULTS: mRNA and protein levels of DNMT3B were found to be elevated in the CAP tissues compared with the control tissue. Additionally, the methylation of long interspersed nuclear elements-1 (LINE-1/L1) was decreased in the CAP tissue. Furthermore, methylation of the promoter of a tumour suppressor gene Ras association domain family 1A (RASSF1A) was increased in the CAP tissues, while the mRNA levels of RASSF1A were decreased. CONCLUSIONS: These results suggest that the overexpression of DNMT3B may contribute to a role in the genesis of CAPs through the hypomethylation of chromosomes in the whole cell and promoter hypermethylation of RASSF1A. Hindawi 2020-10-01 /pmc/articles/PMC7547352/ /pubmed/33061956 http://dx.doi.org/10.1155/2020/1798729 Text en Copyright © 2020 Xianmei Meng et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Meng, Xianmei Liu, Na Jia, Yanbin Gong, Kerui Zhang, Jingjie Zhang, Wei Shao, Guo Dang, Tong DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title_full | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title_fullStr | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title_full_unstemmed | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title_short | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
title_sort | dnmt3b expression might contribute to abnormal methylation of rassf1a in lager colorectal adenomatous polyps |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547352/ https://www.ncbi.nlm.nih.gov/pubmed/33061956 http://dx.doi.org/10.1155/2020/1798729 |
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