Cargando…
Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample
[Image: see text] A disposable electrochemical test strip for the quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNTs) and screen...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2020
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547857/ https://www.ncbi.nlm.nih.gov/pubmed/32842738 http://dx.doi.org/10.1021/acs.analchem.0c01857 |
_version_ | 1783592507980906496 |
---|---|
author | Wester, Niklas Mikladal, Bjørn F. Varjos, Ilkka Peltonen, Antti Kalso, Eija Lilius, Tuomas Laurila, Tomi Koskinen, Jari |
author_facet | Wester, Niklas Mikladal, Bjørn F. Varjos, Ilkka Peltonen, Antti Kalso, Eija Lilius, Tuomas Laurila, Tomi Koskinen, Jari |
author_sort | Wester, Niklas |
collection | PubMed |
description | [Image: see text] A disposable electrochemical test strip for the quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNTs) and screen printing of silver were combined to produce integrated electrochemical test strips. Nafion coating stabilized the potential of the Ag reference electrode and enabled the selective detection in spiked plasma as well as in whole blood samples. The test strips were able to detect acetaminophen in small 40 μL samples with a detection limit of 0.8 μM and a wide linear range from 1 μM to 2 mM, well within the required clinical range. After a simple 1:1 dilution of plasma and whole blood, a quantitative detection with good recoveries of 79% in plasma and 74% in whole blood was achieved. These results strongly indicate that these electrodes can be used directly to determine the unbound acetaminophen fraction without the need for any additional steps. The developed test strip shows promise as a rapid and simple POC quantitative acetaminophen assay. |
format | Online Article Text |
id | pubmed-7547857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-75478572020-10-13 Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample Wester, Niklas Mikladal, Bjørn F. Varjos, Ilkka Peltonen, Antti Kalso, Eija Lilius, Tuomas Laurila, Tomi Koskinen, Jari Anal Chem [Image: see text] A disposable electrochemical test strip for the quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNTs) and screen printing of silver were combined to produce integrated electrochemical test strips. Nafion coating stabilized the potential of the Ag reference electrode and enabled the selective detection in spiked plasma as well as in whole blood samples. The test strips were able to detect acetaminophen in small 40 μL samples with a detection limit of 0.8 μM and a wide linear range from 1 μM to 2 mM, well within the required clinical range. After a simple 1:1 dilution of plasma and whole blood, a quantitative detection with good recoveries of 79% in plasma and 74% in whole blood was achieved. These results strongly indicate that these electrodes can be used directly to determine the unbound acetaminophen fraction without the need for any additional steps. The developed test strip shows promise as a rapid and simple POC quantitative acetaminophen assay. American Chemical Society 2020-08-26 2020-10-06 /pmc/articles/PMC7547857/ /pubmed/32842738 http://dx.doi.org/10.1021/acs.analchem.0c01857 Text en This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Wester, Niklas Mikladal, Bjørn F. Varjos, Ilkka Peltonen, Antti Kalso, Eija Lilius, Tuomas Laurila, Tomi Koskinen, Jari Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample |
title | Disposable Nafion-Coated Single-Walled Carbon Nanotube
Test Strip for Electrochemical Quantitative Determination of Acetaminophen
in a Finger-Prick Whole Blood Sample |
title_full | Disposable Nafion-Coated Single-Walled Carbon Nanotube
Test Strip for Electrochemical Quantitative Determination of Acetaminophen
in a Finger-Prick Whole Blood Sample |
title_fullStr | Disposable Nafion-Coated Single-Walled Carbon Nanotube
Test Strip for Electrochemical Quantitative Determination of Acetaminophen
in a Finger-Prick Whole Blood Sample |
title_full_unstemmed | Disposable Nafion-Coated Single-Walled Carbon Nanotube
Test Strip for Electrochemical Quantitative Determination of Acetaminophen
in a Finger-Prick Whole Blood Sample |
title_short | Disposable Nafion-Coated Single-Walled Carbon Nanotube
Test Strip for Electrochemical Quantitative Determination of Acetaminophen
in a Finger-Prick Whole Blood Sample |
title_sort | disposable nafion-coated single-walled carbon nanotube
test strip for electrochemical quantitative determination of acetaminophen
in a finger-prick whole blood sample |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547857/ https://www.ncbi.nlm.nih.gov/pubmed/32842738 http://dx.doi.org/10.1021/acs.analchem.0c01857 |
work_keys_str_mv | AT westerniklas disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT mikladalbjørnf disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT varjosilkka disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT peltonenantti disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT kalsoeija disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT liliustuomas disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT laurilatomi disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample AT koskinenjari disposablenafioncoatedsinglewalledcarbonnanotubeteststripforelectrochemicalquantitativedeterminationofacetaminopheninafingerprickwholebloodsample |