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Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites

[Image: see text] Here, using human metallothionein (MT2) as an example, we describe an improved strategy based on differential alkylation coupled to MS, assisted by zinc probe monitoring, for identification of cysteine-rich binding sites with nanomolar and picomolar metal affinity utilizing iodoace...

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Detalles Bibliográficos
Autores principales:	Peris-Díaz, Manuel David, Guran, Roman, Zitka, Ondrej, Adam, Vojtech, Krężel, Artur
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2020
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547867/ https://www.ncbi.nlm.nih.gov/pubmed/32786475 http://dx.doi.org/10.1021/acs.analchem.0c01604

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