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The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19

BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We perf...

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Autores principales: Tang, Hao, Gao, Yuehan, Li, Zhaohuai, Miao, Yushan, Huang, Zhaohao, Liu, Xiuxing, Xie, Lihui, Li, He, Wen, Wen, Zheng, Yingfeng, Su, Wenru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548099/
https://www.ncbi.nlm.nih.gov/pubmed/33135345
http://dx.doi.org/10.1002/ctm2.200
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author Tang, Hao
Gao, Yuehan
Li, Zhaohuai
Miao, Yushan
Huang, Zhaohao
Liu, Xiuxing
Xie, Lihui
Li, He
Wen, Wen
Zheng, Yingfeng
Su, Wenru
author_facet Tang, Hao
Gao, Yuehan
Li, Zhaohuai
Miao, Yushan
Huang, Zhaohao
Liu, Xiuxing
Xie, Lihui
Li, He
Wen, Wen
Zheng, Yingfeng
Su, Wenru
author_sort Tang, Hao
collection PubMed
description BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We performed multi‐transcriptome sequencing of both noncoding RNAs and mRNAs isolated from the red blood cell depleted whole blood of moderate and severe COVID‐19 patients. The functions of noncoding RNAs were validated by analyses of the expression of downstream mRNAs. We further utilized the single‐cell RNA‐seq data of COVID‐19 patients from Wilk et al. and Chua et al. to characterize noncoding RNA functions in different cell types. RESULTS: We defined four types of microRNAs with different expression tendencies that could serve as biomarkers for COVID‐19 progress. We also identified miR‐146a‐5p, miR‐21‐5p, miR‐142‐3p, and miR‐15b‐5p as potential contributors to the disease pathogenesis, possibly serving as biomarkers of severe COVID‐19 and as candidate therapeutic targets. In addition, the transcriptome profiles consistently suggested hyperactivation of the immune response, loss of T‐cell function, and immune dysregulation in severe patients. CONCLUSIONS: Collectively, these findings provide a comprehensive view of the noncoding and coding transcriptional landscape of peripheral immune cells during COVID‐19, furthering our understanding and offering novel insights into COVID‐19 pathogenesis.
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spelling pubmed-75480992020-10-16 The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 Tang, Hao Gao, Yuehan Li, Zhaohuai Miao, Yushan Huang, Zhaohao Liu, Xiuxing Xie, Lihui Li, He Wen, Wen Zheng, Yingfeng Su, Wenru Clin Transl Med Research Articles BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We performed multi‐transcriptome sequencing of both noncoding RNAs and mRNAs isolated from the red blood cell depleted whole blood of moderate and severe COVID‐19 patients. The functions of noncoding RNAs were validated by analyses of the expression of downstream mRNAs. We further utilized the single‐cell RNA‐seq data of COVID‐19 patients from Wilk et al. and Chua et al. to characterize noncoding RNA functions in different cell types. RESULTS: We defined four types of microRNAs with different expression tendencies that could serve as biomarkers for COVID‐19 progress. We also identified miR‐146a‐5p, miR‐21‐5p, miR‐142‐3p, and miR‐15b‐5p as potential contributors to the disease pathogenesis, possibly serving as biomarkers of severe COVID‐19 and as candidate therapeutic targets. In addition, the transcriptome profiles consistently suggested hyperactivation of the immune response, loss of T‐cell function, and immune dysregulation in severe patients. CONCLUSIONS: Collectively, these findings provide a comprehensive view of the noncoding and coding transcriptional landscape of peripheral immune cells during COVID‐19, furthering our understanding and offering novel insights into COVID‐19 pathogenesis. John Wiley and Sons Inc. 2020-10-11 /pmc/articles/PMC7548099/ /pubmed/33135345 http://dx.doi.org/10.1002/ctm2.200 Text en © 2020 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Tang, Hao
Gao, Yuehan
Li, Zhaohuai
Miao, Yushan
Huang, Zhaohao
Liu, Xiuxing
Xie, Lihui
Li, He
Wen, Wen
Zheng, Yingfeng
Su, Wenru
The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title_full The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title_fullStr The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title_full_unstemmed The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title_short The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
title_sort noncoding and coding transcriptional landscape of the peripheral immune response in patients with covid‐19
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548099/
https://www.ncbi.nlm.nih.gov/pubmed/33135345
http://dx.doi.org/10.1002/ctm2.200
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