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The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19
BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We perf...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548099/ https://www.ncbi.nlm.nih.gov/pubmed/33135345 http://dx.doi.org/10.1002/ctm2.200 |
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author | Tang, Hao Gao, Yuehan Li, Zhaohuai Miao, Yushan Huang, Zhaohao Liu, Xiuxing Xie, Lihui Li, He Wen, Wen Zheng, Yingfeng Su, Wenru |
author_facet | Tang, Hao Gao, Yuehan Li, Zhaohuai Miao, Yushan Huang, Zhaohao Liu, Xiuxing Xie, Lihui Li, He Wen, Wen Zheng, Yingfeng Su, Wenru |
author_sort | Tang, Hao |
collection | PubMed |
description | BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We performed multi‐transcriptome sequencing of both noncoding RNAs and mRNAs isolated from the red blood cell depleted whole blood of moderate and severe COVID‐19 patients. The functions of noncoding RNAs were validated by analyses of the expression of downstream mRNAs. We further utilized the single‐cell RNA‐seq data of COVID‐19 patients from Wilk et al. and Chua et al. to characterize noncoding RNA functions in different cell types. RESULTS: We defined four types of microRNAs with different expression tendencies that could serve as biomarkers for COVID‐19 progress. We also identified miR‐146a‐5p, miR‐21‐5p, miR‐142‐3p, and miR‐15b‐5p as potential contributors to the disease pathogenesis, possibly serving as biomarkers of severe COVID‐19 and as candidate therapeutic targets. In addition, the transcriptome profiles consistently suggested hyperactivation of the immune response, loss of T‐cell function, and immune dysregulation in severe patients. CONCLUSIONS: Collectively, these findings provide a comprehensive view of the noncoding and coding transcriptional landscape of peripheral immune cells during COVID‐19, furthering our understanding and offering novel insights into COVID‐19 pathogenesis. |
format | Online Article Text |
id | pubmed-7548099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75480992020-10-16 The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 Tang, Hao Gao, Yuehan Li, Zhaohuai Miao, Yushan Huang, Zhaohao Liu, Xiuxing Xie, Lihui Li, He Wen, Wen Zheng, Yingfeng Su, Wenru Clin Transl Med Research Articles BACKGROUND: COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. METHODS: We performed multi‐transcriptome sequencing of both noncoding RNAs and mRNAs isolated from the red blood cell depleted whole blood of moderate and severe COVID‐19 patients. The functions of noncoding RNAs were validated by analyses of the expression of downstream mRNAs. We further utilized the single‐cell RNA‐seq data of COVID‐19 patients from Wilk et al. and Chua et al. to characterize noncoding RNA functions in different cell types. RESULTS: We defined four types of microRNAs with different expression tendencies that could serve as biomarkers for COVID‐19 progress. We also identified miR‐146a‐5p, miR‐21‐5p, miR‐142‐3p, and miR‐15b‐5p as potential contributors to the disease pathogenesis, possibly serving as biomarkers of severe COVID‐19 and as candidate therapeutic targets. In addition, the transcriptome profiles consistently suggested hyperactivation of the immune response, loss of T‐cell function, and immune dysregulation in severe patients. CONCLUSIONS: Collectively, these findings provide a comprehensive view of the noncoding and coding transcriptional landscape of peripheral immune cells during COVID‐19, furthering our understanding and offering novel insights into COVID‐19 pathogenesis. John Wiley and Sons Inc. 2020-10-11 /pmc/articles/PMC7548099/ /pubmed/33135345 http://dx.doi.org/10.1002/ctm2.200 Text en © 2020 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Tang, Hao Gao, Yuehan Li, Zhaohuai Miao, Yushan Huang, Zhaohao Liu, Xiuxing Xie, Lihui Li, He Wen, Wen Zheng, Yingfeng Su, Wenru The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title | The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title_full | The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title_fullStr | The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title_full_unstemmed | The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title_short | The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19 |
title_sort | noncoding and coding transcriptional landscape of the peripheral immune response in patients with covid‐19 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548099/ https://www.ncbi.nlm.nih.gov/pubmed/33135345 http://dx.doi.org/10.1002/ctm2.200 |
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