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Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species
BACKGROUND: Desiccation tolerant Selaginella species evolved to survive extreme environmental conditions. Studies to determine the mechanisms involved in the acquisition of desiccation tolerance (DT) have focused on only a few Selaginella species. Due to the large diversity in morphology and the wid...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549206/ https://www.ncbi.nlm.nih.gov/pubmed/33046015 http://dx.doi.org/10.1186/s12870-020-02638-3 |
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author | Alejo-Jacuinde, Gerardo González-Morales, Sandra Isabel Oropeza-Aburto, Araceli Simpson, June Herrera-Estrella, Luis |
author_facet | Alejo-Jacuinde, Gerardo González-Morales, Sandra Isabel Oropeza-Aburto, Araceli Simpson, June Herrera-Estrella, Luis |
author_sort | Alejo-Jacuinde, Gerardo |
collection | PubMed |
description | BACKGROUND: Desiccation tolerant Selaginella species evolved to survive extreme environmental conditions. Studies to determine the mechanisms involved in the acquisition of desiccation tolerance (DT) have focused on only a few Selaginella species. Due to the large diversity in morphology and the wide range of responses to desiccation within the genus, the understanding of the molecular basis of DT in Selaginella species is still limited. RESULTS: Here we present a reference transcriptome for the desiccation tolerant species S. sellowii and the desiccation sensitive species S. denticulata. The analysis also included transcriptome data for the well-studied S. lepidophylla (desiccation tolerant), in order to identify DT mechanisms that are independent of morphological adaptations. We used a comparative approach to discriminate between DT responses and the common water loss response in Selaginella species. Predicted proteomes show strong homology, but most of the desiccation responsive genes differ between species. Despite such differences, functional analysis revealed that tolerant species with different morphologies employ similar mechanisms to survive desiccation. Significant functions involved in DT and shared by both tolerant species included induction of antioxidant systems, amino acid and secondary metabolism, whereas species-specific responses included cell wall modification and carbohydrate metabolism. CONCLUSIONS: Reference transcriptomes generated in this work represent a valuable resource to study Selaginella biology and plant evolution in relation to DT. Our results provide evidence of convergent evolution of S. sellowii and S. lepidophylla due to the different gene sets that underwent selection to acquire DT |
format | Online Article Text |
id | pubmed-7549206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75492062020-10-13 Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species Alejo-Jacuinde, Gerardo González-Morales, Sandra Isabel Oropeza-Aburto, Araceli Simpson, June Herrera-Estrella, Luis BMC Plant Biol Research Article BACKGROUND: Desiccation tolerant Selaginella species evolved to survive extreme environmental conditions. Studies to determine the mechanisms involved in the acquisition of desiccation tolerance (DT) have focused on only a few Selaginella species. Due to the large diversity in morphology and the wide range of responses to desiccation within the genus, the understanding of the molecular basis of DT in Selaginella species is still limited. RESULTS: Here we present a reference transcriptome for the desiccation tolerant species S. sellowii and the desiccation sensitive species S. denticulata. The analysis also included transcriptome data for the well-studied S. lepidophylla (desiccation tolerant), in order to identify DT mechanisms that are independent of morphological adaptations. We used a comparative approach to discriminate between DT responses and the common water loss response in Selaginella species. Predicted proteomes show strong homology, but most of the desiccation responsive genes differ between species. Despite such differences, functional analysis revealed that tolerant species with different morphologies employ similar mechanisms to survive desiccation. Significant functions involved in DT and shared by both tolerant species included induction of antioxidant systems, amino acid and secondary metabolism, whereas species-specific responses included cell wall modification and carbohydrate metabolism. CONCLUSIONS: Reference transcriptomes generated in this work represent a valuable resource to study Selaginella biology and plant evolution in relation to DT. Our results provide evidence of convergent evolution of S. sellowii and S. lepidophylla due to the different gene sets that underwent selection to acquire DT BioMed Central 2020-10-12 /pmc/articles/PMC7549206/ /pubmed/33046015 http://dx.doi.org/10.1186/s12870-020-02638-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Alejo-Jacuinde, Gerardo González-Morales, Sandra Isabel Oropeza-Aburto, Araceli Simpson, June Herrera-Estrella, Luis Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title | Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title_full | Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title_fullStr | Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title_full_unstemmed | Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title_short | Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species |
title_sort | comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in selaginella species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549206/ https://www.ncbi.nlm.nih.gov/pubmed/33046015 http://dx.doi.org/10.1186/s12870-020-02638-3 |
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