Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed

Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Mayer, Florian J., Ratzinger, Franz, Schmidt, Ralf L.J., Greiner, Georg, Landt, Olfert, Am Ende, Alexander, Corman, Victor M., Perkmann-Nagele, Nicole, Watkins-Riedel, Thomas, Petermann, Dagmar, Abadir, Karoline, Zweimüller-Mayer, Josef, Strassl, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549918/
https://www.ncbi.nlm.nih.gov/pubmed/32726172
http://dx.doi.org/10.1080/21505594.2020.1798041
Descripción
Sumario:Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.

Ejemplares similares