Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed

Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Mayer, Florian J., Ratzinger, Franz, Schmidt, Ralf L.J., Greiner, Georg, Landt, Olfert, Am Ende, Alexander, Corman, Victor M., Perkmann-Nagele, Nicole, Watkins-Riedel, Thomas, Petermann, Dagmar, Abadir, Karoline, Zweimüller-Mayer, Josef, Strassl, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549918/
https://www.ncbi.nlm.nih.gov/pubmed/32726172
http://dx.doi.org/10.1080/21505594.2020.1798041
_version_ 1783592872534081536
author Mayer, Florian J.
Ratzinger, Franz
Schmidt, Ralf L.J.
Greiner, Georg
Landt, Olfert
Am Ende, Alexander
Corman, Victor M.
Perkmann-Nagele, Nicole
Watkins-Riedel, Thomas
Petermann, Dagmar
Abadir, Karoline
Zweimüller-Mayer, Josef
Strassl, Robert
author_facet Mayer, Florian J.
Ratzinger, Franz
Schmidt, Ralf L.J.
Greiner, Georg
Landt, Olfert
Am Ende, Alexander
Corman, Victor M.
Perkmann-Nagele, Nicole
Watkins-Riedel, Thomas
Petermann, Dagmar
Abadir, Karoline
Zweimüller-Mayer, Josef
Strassl, Robert
author_sort Mayer, Florian J.
collection PubMed
description Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
format Online
Article
Text
id pubmed-7549918
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-75499182020-10-27 Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed Mayer, Florian J. Ratzinger, Franz Schmidt, Ralf L.J. Greiner, Georg Landt, Olfert Am Ende, Alexander Corman, Victor M. Perkmann-Nagele, Nicole Watkins-Riedel, Thomas Petermann, Dagmar Abadir, Karoline Zweimüller-Mayer, Josef Strassl, Robert Virulence Research Paper Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84–97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time. Taylor & Francis 2020-07-29 /pmc/articles/PMC7549918/ /pubmed/32726172 http://dx.doi.org/10.1080/21505594.2020.1798041 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Mayer, Florian J.
Ratzinger, Franz
Schmidt, Ralf L.J.
Greiner, Georg
Landt, Olfert
Am Ende, Alexander
Corman, Victor M.
Perkmann-Nagele, Nicole
Watkins-Riedel, Thomas
Petermann, Dagmar
Abadir, Karoline
Zweimüller-Mayer, Josef
Strassl, Robert
Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_full Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_fullStr Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_full_unstemmed Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_short Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
title_sort development of a fully automated high throughput pcr for the detection of sars-cov-2: the need for speed
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549918/
https://www.ncbi.nlm.nih.gov/pubmed/32726172
http://dx.doi.org/10.1080/21505594.2020.1798041
work_keys_str_mv AT mayerflorianj developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT ratzingerfranz developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT schmidtralflj developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT greinergeorg developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT landtolfert developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT amendealexander developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT cormanvictorm developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT perkmannnagelenicole developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT watkinsriedelthomas developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT petermanndagmar developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT abadirkaroline developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT zweimullermayerjosef developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed
AT strasslrobert developmentofafullyautomatedhighthroughputpcrforthedetectionofsarscov2theneedforspeed

Ejemplares similares