Cargando…

Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic

Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Fei, Wang, Yan, He, Qijian, Wang, Zhaoshuo, Ma, Enyue, Zhu, Shiliang, Yu, Heling, Yin, Huadong, Zhao, Xiaoling, Li, Diyan, Xu, Hengyong, Li, Hua, Zhu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549955/
https://www.ncbi.nlm.nih.gov/pubmed/32799626
http://dx.doi.org/10.1080/21505594.2020.1809323
_version_ 1783592877291470848
author Ye, Fei
Wang, Yan
He, Qijian
Wang, Zhaoshuo
Ma, Enyue
Zhu, Shiliang
Yu, Heling
Yin, Huadong
Zhao, Xiaoling
Li, Diyan
Xu, Hengyong
Li, Hua
Zhu, Qing
author_facet Ye, Fei
Wang, Yan
He, Qijian
Wang, Zhaoshuo
Ma, Enyue
Zhu, Shiliang
Yu, Heling
Yin, Huadong
Zhao, Xiaoling
Li, Diyan
Xu, Hengyong
Li, Hua
Zhu, Qing
author_sort Ye, Fei
collection PubMed
description Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.
format Online
Article
Text
id pubmed-7549955
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-75499552020-10-22 Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic Ye, Fei Wang, Yan He, Qijian Wang, Zhaoshuo Ma, Enyue Zhu, Shiliang Yu, Heling Yin, Huadong Zhao, Xiaoling Li, Diyan Xu, Hengyong Li, Hua Zhu, Qing Virulence Research Paper Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry. Taylor & Francis 2020-08-30 /pmc/articles/PMC7549955/ /pubmed/32799626 http://dx.doi.org/10.1080/21505594.2020.1809323 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Ye, Fei
Wang, Yan
He, Qijian
Wang, Zhaoshuo
Ma, Enyue
Zhu, Shiliang
Yu, Heling
Yin, Huadong
Zhao, Xiaoling
Li, Diyan
Xu, Hengyong
Li, Hua
Zhu, Qing
Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_full Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_fullStr Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_full_unstemmed Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_short Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic
title_sort screening of immune biomarkers in different breeds of chickens infected with j subgroup of avian leukemia virus by proteomic
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549955/
https://www.ncbi.nlm.nih.gov/pubmed/32799626
http://dx.doi.org/10.1080/21505594.2020.1809323
work_keys_str_mv AT yefei screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT wangyan screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT heqijian screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT wangzhaoshuo screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT maenyue screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT zhushiliang screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT yuheling screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT yinhuadong screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT zhaoxiaoling screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT lidiyan screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT xuhengyong screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT lihua screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic
AT zhuqing screeningofimmunebiomarkersindifferentbreedsofchickensinfectedwithjsubgroupofavianleukemiavirusbyproteomic