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Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation

Canine parvovirus (CPV) has been used in cancer control as a drug delivery vehicle or anti-tumor reagent due to its multiple natural advantages. However, potential host cell cycle arrest induced by virus infection may impose a big challenge to CPV associated cancer control as it could prevent host c...

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Autores principales: Dai, Xiaofeng, Zhang, Xuanhao, Miao, Yujie, Han, Peiyu, Zhang, Jianying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549965/
https://www.ncbi.nlm.nih.gov/pubmed/32877289
http://dx.doi.org/10.1080/21505594.2020.1814091
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author Dai, Xiaofeng
Zhang, Xuanhao
Miao, Yujie
Han, Peiyu
Zhang, Jianying
author_facet Dai, Xiaofeng
Zhang, Xuanhao
Miao, Yujie
Han, Peiyu
Zhang, Jianying
author_sort Dai, Xiaofeng
collection PubMed
description Canine parvovirus (CPV) has been used in cancer control as a drug delivery vehicle or anti-tumor reagent due to its multiple natural advantages. However, potential host cell cycle arrest induced by virus infection may impose a big challenge to CPV associated cancer control as it could prevent host cancer cells from undergoing cell lysis and foster them regain viability once the virotherapy was ceased. To explore CPV-induced cell cycle arrest and the underlying mechanism toward improved virotherapeutic design, we focus on epidermal growth factor receptor (EGFR), a cellular receptor interacting with TfR that mediates CPV-host interactions, and alterations on its tyrosine phosphorylation sites in response to CPV infection. We found that CPV could trigger host G1/S cell cycle arrest via the EGFR (Y1086)/p27 and EGFR (Y1068)/STAT3/cyclin D1 axes, and EGFR inhibitor could not reverse this process. Our results contribute to our understandings on the mechanism of CPV-induced host cellular response and can be used in the onco-therapeutic design utilizing CPV by preventing host cancer cells from entering cell cycle arrest.
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spelling pubmed-75499652020-10-22 Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation Dai, Xiaofeng Zhang, Xuanhao Miao, Yujie Han, Peiyu Zhang, Jianying Virulence Research Paper Canine parvovirus (CPV) has been used in cancer control as a drug delivery vehicle or anti-tumor reagent due to its multiple natural advantages. However, potential host cell cycle arrest induced by virus infection may impose a big challenge to CPV associated cancer control as it could prevent host cancer cells from undergoing cell lysis and foster them regain viability once the virotherapy was ceased. To explore CPV-induced cell cycle arrest and the underlying mechanism toward improved virotherapeutic design, we focus on epidermal growth factor receptor (EGFR), a cellular receptor interacting with TfR that mediates CPV-host interactions, and alterations on its tyrosine phosphorylation sites in response to CPV infection. We found that CPV could trigger host G1/S cell cycle arrest via the EGFR (Y1086)/p27 and EGFR (Y1068)/STAT3/cyclin D1 axes, and EGFR inhibitor could not reverse this process. Our results contribute to our understandings on the mechanism of CPV-induced host cellular response and can be used in the onco-therapeutic design utilizing CPV by preventing host cancer cells from entering cell cycle arrest. Taylor & Francis 2020-09-02 /pmc/articles/PMC7549965/ /pubmed/32877289 http://dx.doi.org/10.1080/21505594.2020.1814091 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Dai, Xiaofeng
Zhang, Xuanhao
Miao, Yujie
Han, Peiyu
Zhang, Jianying
Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title_full Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title_fullStr Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title_full_unstemmed Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title_short Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
title_sort canine parvovirus induces g1/s cell cycle arrest that involves egfr tyr1086 phosphorylation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549965/
https://www.ncbi.nlm.nih.gov/pubmed/32877289
http://dx.doi.org/10.1080/21505594.2020.1814091
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