Cargando…

Positive control synthesis method for COVID-19 diagnosis by one-step real-time RT-PCR

BACKGROUNDS: The coronavirus disease 2019 (COVID-19) pandemic is still ongoing. Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) is regarded as a gold-standard method for the diagnosis of COVID-19. However, unexpected contamination of synthesized positive control samples...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cho, Hyejin, Jung, Young Hwan, Cho, Hong Bum, Kim, Hee-tae, Kim, Kwang-sun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550048/ https://www.ncbi.nlm.nih.gov/pubmed/33058837 http://dx.doi.org/10.1016/j.cca.2020.10.001

Ejemplares similares

Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
por: Jalal, Sehrish, et al.
Publicado: (2021)

Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR
por: Choudhary, Manohar L., et al.
Publicado: (2013)

One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples
por: Alm, Erik, et al.
Publicado: (2015)

Detection of Langat virus by TaqMan real-time one-step qRT-PCR method
por: Muhd Radzi, Siti Fatimah, et al.
Publicado: (2015)

Development of a one step real time RT-PCR assay to detect and quantify Dugbe virus
por: Rodrigues, R., et al.
Publicado: (2011)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)
por: Jeong, Jae-Kyo, et al.
Publicado: (2014)

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR
por: Zhang, Ni, et al.
Publicado: (2011)

One-Step RT-qPCR for Viral RNA Detection Using Digital Analysis
por: Park, Hyuna, et al.
Publicado: (2022)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus
por: Fu, Wei-Lin, et al.
Publicado: (2015)

Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR
por: Keyaerts, Els, et al.
Publicado: (2006)

Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved in the circadian rhythm using microparticles
por: Kim, Mi Yeon, et al.
Publicado: (2021)

Comparison of the genexpert enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis
por: Hong, JiYoung, et al.
Publicado: (2015)

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil
por: Yang, Jin-Guang, et al.
Publicado: (2012)

The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting Rotavirus A and Norovirus
por: Dung, Tran Thi Ngoc, et al.
Publicado: (2013)

Detection of HEV RNA Using One-Step Real-Time RT-PCR in Farrow-to-Finish Pig Farms in Bulgaria
por: Krumova-Valcheva, Gergana Lyubomirova, et al.
Publicado: (2023)

Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization
por: Manuel, Clyde S., et al.
Publicado: (2021)

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
por: Poon, Leo L.M, et al.
Publicado: (2004)

COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity?
por: Suri, Tejas, et al.
Publicado: (2020)

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2
por: Dharavath, Bhasker, et al.
Publicado: (2020)

Comparison of ordinary reverse transcription real-time polymerase chain reaction (qRT-PCR) with a newly developed one-step single-tube nested real-time RT-PCR (OSN-qRT-PCR) for sensitive detection of SARS-CoV-2 in wastewater
por: Rusková, Magdaléna, et al.
Publicado: (2023)

An Open One-Step RT-qPCR for SARS-CoV-2 detection
por: Cerda, Ariel, et al.
Publicado: (2023)

The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus
por: Tian, Hong, et al.
Publicado: (2010)

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
por: LIU, Dafei, et al.
Publicado: (2018)

One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3
por: Thonur, Leenadevi, et al.
Publicado: (2012)

Early diagnosis of SARS Coronavirus infection by real time RT-PCR
por: Poon, Leo L.M., et al.
Publicado: (2003)

Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects
por: Sule, Waidi Folorunso, et al.
Publicado: (2020)

Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe
por: Hong, JiYoung, et al.
Publicado: (2011)

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
por: Hanaki, Ken-Ichi, et al.
Publicado: (2014)

Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses
por: Pang, Zheng, et al.
Publicado: (2014)

A novel multiplex one-step real-time RT-PCR assay for the simultaneous identification of enterovirus and parechovirus in clinical fecal samples
por: BUBBA, L., et al.
Publicado: (2015)

Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms
por: You, Huey-Ling, et al.
Publicado: (2017)

Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR
por: Bigault, Lionel, et al.
Publicado: (2020)

Genetic diversity of porcine reproductive and respiratory syndrome virus and evaluation of three one-step real-time RT-PCR assays in Korea
por: Shin, Go-Eun, et al.
Publicado: (2022)

One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses
por: Rački, Nejc, et al.
Publicado: (2013)

Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
por: Chi, X. Sherry, et al.
Publicado: (2007)

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus
por: Dedkov, Vladimir G., et al.
Publicado: (2019)

Detection of avian influenza virus and newcastle disease virus by duplex one step RT PCR
por: Nidzworski, Dawid, et al.
Publicado: (2013)

P.069 Comparison of one and two step RT-PCR in the detection of RNA respiratory viruses
por: Tilston, P., et al.
Publicado: (2009)

Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection
por: Malekshahi, Asra, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_mhfm1fo79l7pb75ic9lsona6fr