Cargando…

Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence

BACKGROUND: The purpose of this paper was to examine the effects of nicotinamide (ND) and l-arginine (l-ARG) on pulmonary vascular and heart changes induced by pulmonary hypertension in rats in a gender-dependent way. METHODS: Experiments were performed on male (M) and female (F) rats. PAH was induc...

Descripción completa

Detalles Bibliográficos
Autores principales: Sztormowska-Achranowicz, Katarzyna, Jankowski, Zbigniew, Kocić, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550290/
https://www.ncbi.nlm.nih.gov/pubmed/32632916
http://dx.doi.org/10.1007/s43440-020-00125-y
_version_ 1783592943948398592
author Sztormowska-Achranowicz, Katarzyna
Jankowski, Zbigniew
Kocić, Ivan
author_facet Sztormowska-Achranowicz, Katarzyna
Jankowski, Zbigniew
Kocić, Ivan
author_sort Sztormowska-Achranowicz, Katarzyna
collection PubMed
description BACKGROUND: The purpose of this paper was to examine the effects of nicotinamide (ND) and l-arginine (l-ARG) on pulmonary vascular and heart changes induced by pulmonary hypertension in rats in a gender-dependent way. METHODS: Experiments were performed on male (M) and female (F) rats. PAH was induced via monocrotaline injection (sc, 60/kg B.W.) on day one of the 23-day observational period. After that, the animals were sacrificed, hearts removed and weighed and the papillary muscles isolated to measure force of contraction (F(c)). Morphological changes of pulmonary vessels were also examined. RESULTS: Mixed diet supplementation with l-ARG + ND prevented highly significant right ventricle enlargement induced by PAH in both, male and female rats. Weight ratios between the right ventricle (RV) on one side and the left ventricle with septum on the other (LV + S) decreased from 0.46 ± 0.016 g to 0.29 ± 0.006 g in males and from 0.63 ± 0.03 g to 0.24 ± 0.008 g in females, n = 6, p < 0.001. Additionally, PAH increased basal contractility in female groups, and each of the diet allocations (l-ARG, ND, and mixed) were found to restore contractility to control values. All diet protocols in male and female restored decreased responsiveness of the myocardium to norepinephrine in hearts obtained from rats with PAH and prevented vascular changes observed in pulmonary hypertension (thickness of blood vessels and cell infiltration). CONCLUSION: Our study suggests that l-arginine, nicotinamide or both play a positive role in right ventricle function or the process reducing pulmonary vascular remodeling especially in a gender-independent way.
format Online
Article
Text
id pubmed-7550290
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-75502902020-10-19 Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence Sztormowska-Achranowicz, Katarzyna Jankowski, Zbigniew Kocić, Ivan Pharmacol Rep Article BACKGROUND: The purpose of this paper was to examine the effects of nicotinamide (ND) and l-arginine (l-ARG) on pulmonary vascular and heart changes induced by pulmonary hypertension in rats in a gender-dependent way. METHODS: Experiments were performed on male (M) and female (F) rats. PAH was induced via monocrotaline injection (sc, 60/kg B.W.) on day one of the 23-day observational period. After that, the animals were sacrificed, hearts removed and weighed and the papillary muscles isolated to measure force of contraction (F(c)). Morphological changes of pulmonary vessels were also examined. RESULTS: Mixed diet supplementation with l-ARG + ND prevented highly significant right ventricle enlargement induced by PAH in both, male and female rats. Weight ratios between the right ventricle (RV) on one side and the left ventricle with septum on the other (LV + S) decreased from 0.46 ± 0.016 g to 0.29 ± 0.006 g in males and from 0.63 ± 0.03 g to 0.24 ± 0.008 g in females, n = 6, p < 0.001. Additionally, PAH increased basal contractility in female groups, and each of the diet allocations (l-ARG, ND, and mixed) were found to restore contractility to control values. All diet protocols in male and female restored decreased responsiveness of the myocardium to norepinephrine in hearts obtained from rats with PAH and prevented vascular changes observed in pulmonary hypertension (thickness of blood vessels and cell infiltration). CONCLUSION: Our study suggests that l-arginine, nicotinamide or both play a positive role in right ventricle function or the process reducing pulmonary vascular remodeling especially in a gender-independent way. Springer International Publishing 2020-07-06 2020 /pmc/articles/PMC7550290/ /pubmed/32632916 http://dx.doi.org/10.1007/s43440-020-00125-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sztormowska-Achranowicz, Katarzyna
Jankowski, Zbigniew
Kocić, Ivan
Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title_full Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title_fullStr Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title_full_unstemmed Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title_short Protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
title_sort protective effect of nicotinamide and l-arginine against monocrotaline-induced pulmonary hypertension in rats: gender dependence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550290/
https://www.ncbi.nlm.nih.gov/pubmed/32632916
http://dx.doi.org/10.1007/s43440-020-00125-y
work_keys_str_mv AT sztormowskaachranowiczkatarzyna protectiveeffectofnicotinamideandlarginineagainstmonocrotalineinducedpulmonaryhypertensioninratsgenderdependence
AT jankowskizbigniew protectiveeffectofnicotinamideandlarginineagainstmonocrotalineinducedpulmonaryhypertensioninratsgenderdependence
AT kocicivan protectiveeffectofnicotinamideandlarginineagainstmonocrotalineinducedpulmonaryhypertensioninratsgenderdependence