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Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide

Pseudomonas syringae is a phytopathogenic model bacterium that is used worldwide to study plant–bacteria interactions and biofilm formation in association with a plant host. Within this species, the syringae pathovar is the most studied due to its wide host range, affecting both, woody and herbaceou...

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Autores principales: Heredia-Ponce, Zaira, Gutiérrez-Barranquero, Jose Antonio, Purtschert-Montenegro, Gabriela, Eberl, Leo, Cazorla, Francisco M., de Vicente, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550585/
https://www.ncbi.nlm.nih.gov/pubmed/33046713
http://dx.doi.org/10.1038/s41522-020-00148-6
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author Heredia-Ponce, Zaira
Gutiérrez-Barranquero, Jose Antonio
Purtschert-Montenegro, Gabriela
Eberl, Leo
Cazorla, Francisco M.
de Vicente, Antonio
author_facet Heredia-Ponce, Zaira
Gutiérrez-Barranquero, Jose Antonio
Purtschert-Montenegro, Gabriela
Eberl, Leo
Cazorla, Francisco M.
de Vicente, Antonio
author_sort Heredia-Ponce, Zaira
collection PubMed
description Pseudomonas syringae is a phytopathogenic model bacterium that is used worldwide to study plant–bacteria interactions and biofilm formation in association with a plant host. Within this species, the syringae pathovar is the most studied due to its wide host range, affecting both, woody and herbaceous plants. In particular, Pseudomonas syringae pv. syringae (Pss) has been previously described as the causal agent of bacterial apical necrosis on mango trees. Pss exhibits major epiphytic traits and virulence factors that improve its epiphytic survival and pathogenicity in mango trees. The cellulose exopolysaccharide has been described as a key component in the development of the biofilm lifestyle of the P. syringae pv. syringae UMAF0158 strain (PssUMAF0158). PssUMAF0158 contains two additional genomic regions that putatively encode for exopolysaccharides such as alginate and a Psl-like polysaccharide. To date, the Psl polysaccharide has only been studied in Pseudomonas aeruginosa, in which it plays an important role during biofilm development. However, its function in plant-associated bacteria is still unknown. To understand how these exopolysaccharides contribute to the biofilm matrix of PssUMAF0158, knockout mutants of genes encoding these putative exopolysaccharides were constructed. Flow-cell chamber experiments revealed that cellulose and the Psl-like polysaccharide constitute a basic scaffold for biofilm architecture in this bacterium. Curiously, the Psl-like polysaccharide of PssUMAF0158 plays a role in virulence similar to what has been described for cellulose. Finally, the impaired swarming motility of the Psl-like exopolysaccharide mutant suggests that this exopolysaccharide may play a role in the motility of PssUMAF0158 over the mango plant surface.
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spelling pubmed-75505852020-10-19 Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide Heredia-Ponce, Zaira Gutiérrez-Barranquero, Jose Antonio Purtschert-Montenegro, Gabriela Eberl, Leo Cazorla, Francisco M. de Vicente, Antonio NPJ Biofilms Microbiomes Article Pseudomonas syringae is a phytopathogenic model bacterium that is used worldwide to study plant–bacteria interactions and biofilm formation in association with a plant host. Within this species, the syringae pathovar is the most studied due to its wide host range, affecting both, woody and herbaceous plants. In particular, Pseudomonas syringae pv. syringae (Pss) has been previously described as the causal agent of bacterial apical necrosis on mango trees. Pss exhibits major epiphytic traits and virulence factors that improve its epiphytic survival and pathogenicity in mango trees. The cellulose exopolysaccharide has been described as a key component in the development of the biofilm lifestyle of the P. syringae pv. syringae UMAF0158 strain (PssUMAF0158). PssUMAF0158 contains two additional genomic regions that putatively encode for exopolysaccharides such as alginate and a Psl-like polysaccharide. To date, the Psl polysaccharide has only been studied in Pseudomonas aeruginosa, in which it plays an important role during biofilm development. However, its function in plant-associated bacteria is still unknown. To understand how these exopolysaccharides contribute to the biofilm matrix of PssUMAF0158, knockout mutants of genes encoding these putative exopolysaccharides were constructed. Flow-cell chamber experiments revealed that cellulose and the Psl-like polysaccharide constitute a basic scaffold for biofilm architecture in this bacterium. Curiously, the Psl-like polysaccharide of PssUMAF0158 plays a role in virulence similar to what has been described for cellulose. Finally, the impaired swarming motility of the Psl-like exopolysaccharide mutant suggests that this exopolysaccharide may play a role in the motility of PssUMAF0158 over the mango plant surface. Nature Publishing Group UK 2020-10-12 /pmc/articles/PMC7550585/ /pubmed/33046713 http://dx.doi.org/10.1038/s41522-020-00148-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Heredia-Ponce, Zaira
Gutiérrez-Barranquero, Jose Antonio
Purtschert-Montenegro, Gabriela
Eberl, Leo
Cazorla, Francisco M.
de Vicente, Antonio
Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title_full Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title_fullStr Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title_full_unstemmed Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title_short Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
title_sort biological role of eps from pseudomonas syringae pv. syringae umaf0158 extracellular matrix, focusing on a psl-like polysaccharide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550585/
https://www.ncbi.nlm.nih.gov/pubmed/33046713
http://dx.doi.org/10.1038/s41522-020-00148-6
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