Cargando…

Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex

The circadian rhythmicity changes the density and shape of dendritic spines in mouse somatosensory barrel cortex, influencing their stability and maturation. In this study, we analyzed the main geometric parameters of dendritic spines reflecting the strength of synapses located on these spines under...

Descripción completa

Detalles Bibliográficos
Autores principales: Jasinska, Malgorzata, Woznicka, Olga, Jasek-Gajda, Ewa, Lis, Grzegorz J., Pyza, Elzbieta, Litwin, Jan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550732/
https://www.ncbi.nlm.nih.gov/pubmed/33117123
http://dx.doi.org/10.3389/fnins.2020.578881
_version_ 1783593029104304128
author Jasinska, Malgorzata
Woznicka, Olga
Jasek-Gajda, Ewa
Lis, Grzegorz J.
Pyza, Elzbieta
Litwin, Jan A.
author_facet Jasinska, Malgorzata
Woznicka, Olga
Jasek-Gajda, Ewa
Lis, Grzegorz J.
Pyza, Elzbieta
Litwin, Jan A.
author_sort Jasinska, Malgorzata
collection PubMed
description The circadian rhythmicity changes the density and shape of dendritic spines in mouse somatosensory barrel cortex, influencing their stability and maturation. In this study, we analyzed the main geometric parameters of dendritic spines reflecting the strength of synapses located on these spines under light/dark (12:12) and constant darkness conditions, in order to distinguish between endogenously regulated and light-driven parameters. Using morphological analysis of serial electron micrographs, as well as three-dimensional reconstructions, we found that the light induces elongation of single-synapse spine necks and increases in the diameter of double-synapse spine necks, increasing and decreasing the isolation of synapses from the parent dendrite, respectively. During the subjective night of constant darkness, we observed an enlargement of postsynaptic density area in inhibitory synapses and an increase in the number of polyribosomes inside double-synapse spines. The results show that both endogenous effect (circadian clock/locomotor activity) and light affect the morphological parameters of single- and double-synapse spines in the somatosensory cortex: light reduces the efficiency of excitatory synapses on single-synapse spines, increases the effect of synaptic transmission in double-synapse spines, and additionally masks the endogenous clock-driven enlargement of inhibitory synapses located on double-synapse spines. This indicates a special role of double-synapse spines and their inhibitory synapses in the regulation of synaptic transmission during both circadian and diurnal cycles in the mouse somatosensory cortex.
format Online
Article
Text
id pubmed-7550732
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-75507322020-10-27 Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex Jasinska, Malgorzata Woznicka, Olga Jasek-Gajda, Ewa Lis, Grzegorz J. Pyza, Elzbieta Litwin, Jan A. Front Neurosci Neuroscience The circadian rhythmicity changes the density and shape of dendritic spines in mouse somatosensory barrel cortex, influencing their stability and maturation. In this study, we analyzed the main geometric parameters of dendritic spines reflecting the strength of synapses located on these spines under light/dark (12:12) and constant darkness conditions, in order to distinguish between endogenously regulated and light-driven parameters. Using morphological analysis of serial electron micrographs, as well as three-dimensional reconstructions, we found that the light induces elongation of single-synapse spine necks and increases in the diameter of double-synapse spine necks, increasing and decreasing the isolation of synapses from the parent dendrite, respectively. During the subjective night of constant darkness, we observed an enlargement of postsynaptic density area in inhibitory synapses and an increase in the number of polyribosomes inside double-synapse spines. The results show that both endogenous effect (circadian clock/locomotor activity) and light affect the morphological parameters of single- and double-synapse spines in the somatosensory cortex: light reduces the efficiency of excitatory synapses on single-synapse spines, increases the effect of synaptic transmission in double-synapse spines, and additionally masks the endogenous clock-driven enlargement of inhibitory synapses located on double-synapse spines. This indicates a special role of double-synapse spines and their inhibitory synapses in the regulation of synaptic transmission during both circadian and diurnal cycles in the mouse somatosensory cortex. Frontiers Media S.A. 2020-09-29 /pmc/articles/PMC7550732/ /pubmed/33117123 http://dx.doi.org/10.3389/fnins.2020.578881 Text en Copyright © 2020 Jasinska, Woznicka, Jasek-Gajda, Lis, Pyza and Litwin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Jasinska, Malgorzata
Woznicka, Olga
Jasek-Gajda, Ewa
Lis, Grzegorz J.
Pyza, Elzbieta
Litwin, Jan A.
Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title_full Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title_fullStr Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title_full_unstemmed Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title_short Circadian Changes of Dendritic Spine Geometry in Mouse Barrel Cortex
title_sort circadian changes of dendritic spine geometry in mouse barrel cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550732/
https://www.ncbi.nlm.nih.gov/pubmed/33117123
http://dx.doi.org/10.3389/fnins.2020.578881
work_keys_str_mv AT jasinskamalgorzata circadianchangesofdendriticspinegeometryinmousebarrelcortex
AT woznickaolga circadianchangesofdendriticspinegeometryinmousebarrelcortex
AT jasekgajdaewa circadianchangesofdendriticspinegeometryinmousebarrelcortex
AT lisgrzegorzj circadianchangesofdendriticspinegeometryinmousebarrelcortex
AT pyzaelzbieta circadianchangesofdendriticspinegeometryinmousebarrelcortex
AT litwinjana circadianchangesofdendriticspinegeometryinmousebarrelcortex