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An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration
Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual’s quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550851/ https://www.ncbi.nlm.nih.gov/pubmed/33117779 http://dx.doi.org/10.3389/fbioe.2020.559494 |
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author | El-Sayed, Basmah Davies, Robert Philip Wynn El-Zehery, Rehab R. Ibrahim, Fatma Mohamed Grawish, Mohammed E. Kirkham, Jennifer El-Gendy, Reem |
author_facet | El-Sayed, Basmah Davies, Robert Philip Wynn El-Zehery, Rehab R. Ibrahim, Fatma Mohamed Grawish, Mohammed E. Kirkham, Jennifer El-Gendy, Reem |
author_sort | El-Sayed, Basmah |
collection | PubMed |
description | Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual’s quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a step-change in nanobiotechnology and regenerative medicine fields. Our aim was to investigate the effects of a self-assembling peptide (SAP; P(11)-4) on periodontal regeneration in a preclinical model. Twenty-six bilateral maxillary critical-sized periodontal defects were created surgically in 13 rats. Defects on one side of the mouth were filled with P(11)-4 hydrogel; the contra-lateral defect was untreated (control). Rats were sacrificed immediately post-surgery (time 0) and after 2 and 4 weeks. Retrieved maxillae were processed for histological, immunohistochemical, and histomorphometric assessments. The results of histological analysis showed greater organization of periodontal fibers in defects treated with P(11)-4, at both time points, when compared to untreated defects. Histomorphometry showed that treated defects had both a significant increase in functional periodontal ligament length and a reduction in epithelial down growth after 4 weeks. At 2 weeks, treated defects showed a significant increase in expression of osteocalcin and osteoprotegerin as judged by immunohistochemistry. Also, a significantly higher osteoprotegerin/RANKL ratio was shown in treated defects. In conclusion, the results demonstrated enhanced regeneration of periodontal tissues when SAP P(11)-4 was used to fill periodontal defects in rats. The findings of this study suggest that SAP P(11)-4 is a promising novel candidate for periodontal regenerative therapy. Further investigations are required for optimization before clinical use. |
format | Online Article Text |
id | pubmed-7550851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75508512020-10-27 An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration El-Sayed, Basmah Davies, Robert Philip Wynn El-Zehery, Rehab R. Ibrahim, Fatma Mohamed Grawish, Mohammed E. Kirkham, Jennifer El-Gendy, Reem Front Bioeng Biotechnol Bioengineering and Biotechnology Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual’s quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a step-change in nanobiotechnology and regenerative medicine fields. Our aim was to investigate the effects of a self-assembling peptide (SAP; P(11)-4) on periodontal regeneration in a preclinical model. Twenty-six bilateral maxillary critical-sized periodontal defects were created surgically in 13 rats. Defects on one side of the mouth were filled with P(11)-4 hydrogel; the contra-lateral defect was untreated (control). Rats were sacrificed immediately post-surgery (time 0) and after 2 and 4 weeks. Retrieved maxillae were processed for histological, immunohistochemical, and histomorphometric assessments. The results of histological analysis showed greater organization of periodontal fibers in defects treated with P(11)-4, at both time points, when compared to untreated defects. Histomorphometry showed that treated defects had both a significant increase in functional periodontal ligament length and a reduction in epithelial down growth after 4 weeks. At 2 weeks, treated defects showed a significant increase in expression of osteocalcin and osteoprotegerin as judged by immunohistochemistry. Also, a significantly higher osteoprotegerin/RANKL ratio was shown in treated defects. In conclusion, the results demonstrated enhanced regeneration of periodontal tissues when SAP P(11)-4 was used to fill periodontal defects in rats. The findings of this study suggest that SAP P(11)-4 is a promising novel candidate for periodontal regenerative therapy. Further investigations are required for optimization before clinical use. Frontiers Media S.A. 2020-09-23 /pmc/articles/PMC7550851/ /pubmed/33117779 http://dx.doi.org/10.3389/fbioe.2020.559494 Text en Copyright © 2020 El-Sayed, Davies, El-Zehery, Ibrahim, Grawish, Kirkham and El-Gendy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology El-Sayed, Basmah Davies, Robert Philip Wynn El-Zehery, Rehab R. Ibrahim, Fatma Mohamed Grawish, Mohammed E. Kirkham, Jennifer El-Gendy, Reem An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title | An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title_full | An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title_fullStr | An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title_full_unstemmed | An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title_short | An in-vivo Intraoral Defect Model for Assessing the Use of P(11)-4 Self-Assembling Peptide in Periodontal Regeneration |
title_sort | in-vivo intraoral defect model for assessing the use of p(11)-4 self-assembling peptide in periodontal regeneration |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550851/ https://www.ncbi.nlm.nih.gov/pubmed/33117779 http://dx.doi.org/10.3389/fbioe.2020.559494 |
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