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Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction

The relationship between change of gut microbiota and host serum metabolomics associated with low protein diet (LPD) has been unraveled incompletely in CKD patients. Fecal 16S rRNA gene sequencing and serum metabolomics profiling were performed. We reported significant changes in the β-diversity of...

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Autores principales: Wu, I-Wen, Lee, Chin-Chan, Hsu, Heng-Jung, Sun, Chiao-Yin, Chen, Yuen-Chan, Yang, Kai-Jie, Yang, Chi-Wei, Chung, Wen-Hun, Lai, Hsin-Chih, Chang, Lun-Ching, Su, Shih-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551076/
https://www.ncbi.nlm.nih.gov/pubmed/32932711
http://dx.doi.org/10.3390/nu12092799
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author Wu, I-Wen
Lee, Chin-Chan
Hsu, Heng-Jung
Sun, Chiao-Yin
Chen, Yuen-Chan
Yang, Kai-Jie
Yang, Chi-Wei
Chung, Wen-Hun
Lai, Hsin-Chih
Chang, Lun-Ching
Su, Shih-Chi
author_facet Wu, I-Wen
Lee, Chin-Chan
Hsu, Heng-Jung
Sun, Chiao-Yin
Chen, Yuen-Chan
Yang, Kai-Jie
Yang, Chi-Wei
Chung, Wen-Hun
Lai, Hsin-Chih
Chang, Lun-Ching
Su, Shih-Chi
author_sort Wu, I-Wen
collection PubMed
description The relationship between change of gut microbiota and host serum metabolomics associated with low protein diet (LPD) has been unraveled incompletely in CKD patients. Fecal 16S rRNA gene sequencing and serum metabolomics profiling were performed. We reported significant changes in the β-diversity of gut microbiota in CKD patients having LPD (CKD-LPD, n = 16). We identified 19 genera and 12 species with significant differences in their relative abundance among CKD-LPD patients compared to patients receiving normal protein diet (CKD-NPD, n = 27) or non-CKD controls (n = 34), respectively. CKD-LPD had a significant decrease in the abundance of many butyrate-producing bacteria (family Lachnospiraceae and Bacteroidaceae) associated with enrichment of functional module of butanoate metabolism, leading to concomitant reduction in serum levels of SCFA (acetic, heptanoic and nonanoic acid). A secondary bile acid, glyco λ-muricholic acid, was significantly increased in CKD-LPD patients. Serum levels of indoxyl sulfate and p-cresyl sulfate did not differ among groups. The relationship between abundances of microbes and metabolites remained significant in subset of resampling subjects of comparable characteristics. Enrichment of bacterial gene markers related to D-alanine, ketone bodies and glutathione metabolism was noted in CKD-LPD patients. Our analyses reveal signatures and functions of gut microbiota to adapt dietary protein restriction in renal patients.
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spelling pubmed-75510762020-10-16 Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction Wu, I-Wen Lee, Chin-Chan Hsu, Heng-Jung Sun, Chiao-Yin Chen, Yuen-Chan Yang, Kai-Jie Yang, Chi-Wei Chung, Wen-Hun Lai, Hsin-Chih Chang, Lun-Ching Su, Shih-Chi Nutrients Article The relationship between change of gut microbiota and host serum metabolomics associated with low protein diet (LPD) has been unraveled incompletely in CKD patients. Fecal 16S rRNA gene sequencing and serum metabolomics profiling were performed. We reported significant changes in the β-diversity of gut microbiota in CKD patients having LPD (CKD-LPD, n = 16). We identified 19 genera and 12 species with significant differences in their relative abundance among CKD-LPD patients compared to patients receiving normal protein diet (CKD-NPD, n = 27) or non-CKD controls (n = 34), respectively. CKD-LPD had a significant decrease in the abundance of many butyrate-producing bacteria (family Lachnospiraceae and Bacteroidaceae) associated with enrichment of functional module of butanoate metabolism, leading to concomitant reduction in serum levels of SCFA (acetic, heptanoic and nonanoic acid). A secondary bile acid, glyco λ-muricholic acid, was significantly increased in CKD-LPD patients. Serum levels of indoxyl sulfate and p-cresyl sulfate did not differ among groups. The relationship between abundances of microbes and metabolites remained significant in subset of resampling subjects of comparable characteristics. Enrichment of bacterial gene markers related to D-alanine, ketone bodies and glutathione metabolism was noted in CKD-LPD patients. Our analyses reveal signatures and functions of gut microbiota to adapt dietary protein restriction in renal patients. MDPI 2020-09-12 /pmc/articles/PMC7551076/ /pubmed/32932711 http://dx.doi.org/10.3390/nu12092799 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, I-Wen
Lee, Chin-Chan
Hsu, Heng-Jung
Sun, Chiao-Yin
Chen, Yuen-Chan
Yang, Kai-Jie
Yang, Chi-Wei
Chung, Wen-Hun
Lai, Hsin-Chih
Chang, Lun-Ching
Su, Shih-Chi
Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title_full Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title_fullStr Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title_full_unstemmed Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title_short Compositional and Functional Adaptations of Intestinal Microbiota and Related Metabolites in CKD Patients Receiving Dietary Protein Restriction
title_sort compositional and functional adaptations of intestinal microbiota and related metabolites in ckd patients receiving dietary protein restriction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551076/
https://www.ncbi.nlm.nih.gov/pubmed/32932711
http://dx.doi.org/10.3390/nu12092799
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