Cargando…
Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Me...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551274/ https://www.ncbi.nlm.nih.gov/pubmed/32971775 http://dx.doi.org/10.3390/nu12092889 |
_version_ | 1783593147967733760 |
---|---|
author | García-Legorreta, Arantxa Soriano-Pérez, Luis Alfonso Flores-Buendía, Aline Mariana Medina-Campos, Omar Noel Noriega, Lilia G. Granados-Portillo, Omar Nambo-Venegas, Rafael Tovar, Armando R. Mendoza-Vargas, Alfredo Barrera-Oviedo, Diana Pedraza-Chaverri, José Palacios-González, Berenice |
author_facet | García-Legorreta, Arantxa Soriano-Pérez, Luis Alfonso Flores-Buendía, Aline Mariana Medina-Campos, Omar Noel Noriega, Lilia G. Granados-Portillo, Omar Nambo-Venegas, Rafael Tovar, Armando R. Mendoza-Vargas, Alfredo Barrera-Oviedo, Diana Pedraza-Chaverri, José Palacios-González, Berenice |
author_sort | García-Legorreta, Arantxa |
collection | PubMed |
description | Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, Dorea, Lactobacillus and Turibacter were enriched in the L-Mg group. In contrast, the phyla Proteobacteria, Parabacteroides, Butyricimonas, and Victivallis were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet. |
format | Online Article Text |
id | pubmed-7551274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75512742020-10-16 Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats García-Legorreta, Arantxa Soriano-Pérez, Luis Alfonso Flores-Buendía, Aline Mariana Medina-Campos, Omar Noel Noriega, Lilia G. Granados-Portillo, Omar Nambo-Venegas, Rafael Tovar, Armando R. Mendoza-Vargas, Alfredo Barrera-Oviedo, Diana Pedraza-Chaverri, José Palacios-González, Berenice Nutrients Article Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, Dorea, Lactobacillus and Turibacter were enriched in the L-Mg group. In contrast, the phyla Proteobacteria, Parabacteroides, Butyricimonas, and Victivallis were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet. MDPI 2020-09-22 /pmc/articles/PMC7551274/ /pubmed/32971775 http://dx.doi.org/10.3390/nu12092889 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article García-Legorreta, Arantxa Soriano-Pérez, Luis Alfonso Flores-Buendía, Aline Mariana Medina-Campos, Omar Noel Noriega, Lilia G. Granados-Portillo, Omar Nambo-Venegas, Rafael Tovar, Armando R. Mendoza-Vargas, Alfredo Barrera-Oviedo, Diana Pedraza-Chaverri, José Palacios-González, Berenice Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_full | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_fullStr | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_full_unstemmed | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_short | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_sort | effect of dietary magnesium content on intestinal microbiota of rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551274/ https://www.ncbi.nlm.nih.gov/pubmed/32971775 http://dx.doi.org/10.3390/nu12092889 |
work_keys_str_mv | AT garcialegorretaarantxa effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT sorianoperezluisalfonso effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT floresbuendiaalinemariana effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT medinacamposomarnoel effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT noriegaliliag effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT granadosportilloomar effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT nambovenegasrafael effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT tovararmandor effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT mendozavargasalfredo effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT barreraoviedodiana effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT pedrazachaverrijose effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats AT palaciosgonzalezberenice effectofdietarymagnesiumcontentonintestinalmicrobiotaofrats |