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Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats

Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Me...

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Autores principales: García-Legorreta, Arantxa, Soriano-Pérez, Luis Alfonso, Flores-Buendía, Aline Mariana, Medina-Campos, Omar Noel, Noriega, Lilia G., Granados-Portillo, Omar, Nambo-Venegas, Rafael, Tovar, Armando R., Mendoza-Vargas, Alfredo, Barrera-Oviedo, Diana, Pedraza-Chaverri, José, Palacios-González, Berenice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551274/
https://www.ncbi.nlm.nih.gov/pubmed/32971775
http://dx.doi.org/10.3390/nu12092889
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author García-Legorreta, Arantxa
Soriano-Pérez, Luis Alfonso
Flores-Buendía, Aline Mariana
Medina-Campos, Omar Noel
Noriega, Lilia G.
Granados-Portillo, Omar
Nambo-Venegas, Rafael
Tovar, Armando R.
Mendoza-Vargas, Alfredo
Barrera-Oviedo, Diana
Pedraza-Chaverri, José
Palacios-González, Berenice
author_facet García-Legorreta, Arantxa
Soriano-Pérez, Luis Alfonso
Flores-Buendía, Aline Mariana
Medina-Campos, Omar Noel
Noriega, Lilia G.
Granados-Portillo, Omar
Nambo-Venegas, Rafael
Tovar, Armando R.
Mendoza-Vargas, Alfredo
Barrera-Oviedo, Diana
Pedraza-Chaverri, José
Palacios-González, Berenice
author_sort García-Legorreta, Arantxa
collection PubMed
description Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, Dorea, Lactobacillus and Turibacter were enriched in the L-Mg group. In contrast, the phyla Proteobacteria, Parabacteroides, Butyricimonas, and Victivallis were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet.
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spelling pubmed-75512742020-10-16 Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats García-Legorreta, Arantxa Soriano-Pérez, Luis Alfonso Flores-Buendía, Aline Mariana Medina-Campos, Omar Noel Noriega, Lilia G. Granados-Portillo, Omar Nambo-Venegas, Rafael Tovar, Armando R. Mendoza-Vargas, Alfredo Barrera-Oviedo, Diana Pedraza-Chaverri, José Palacios-González, Berenice Nutrients Article Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, Dorea, Lactobacillus and Turibacter were enriched in the L-Mg group. In contrast, the phyla Proteobacteria, Parabacteroides, Butyricimonas, and Victivallis were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet. MDPI 2020-09-22 /pmc/articles/PMC7551274/ /pubmed/32971775 http://dx.doi.org/10.3390/nu12092889 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Legorreta, Arantxa
Soriano-Pérez, Luis Alfonso
Flores-Buendía, Aline Mariana
Medina-Campos, Omar Noel
Noriega, Lilia G.
Granados-Portillo, Omar
Nambo-Venegas, Rafael
Tovar, Armando R.
Mendoza-Vargas, Alfredo
Barrera-Oviedo, Diana
Pedraza-Chaverri, José
Palacios-González, Berenice
Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_full Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_fullStr Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_full_unstemmed Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_short Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_sort effect of dietary magnesium content on intestinal microbiota of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551274/
https://www.ncbi.nlm.nih.gov/pubmed/32971775
http://dx.doi.org/10.3390/nu12092889
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