Cargando…
Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin
Prenatal leptin is key to regulating foetal growth and early metabolic programming. The presence of intact leptin in rat foetal (at late gestation) and neonatal (immediately after birth) stomach content and mucosa has been previously described, suggesting that it may act as a regulatory nutrient for...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551401/ https://www.ncbi.nlm.nih.gov/pubmed/32825787 http://dx.doi.org/10.3390/nu12092542 |
_version_ | 1783593176079007744 |
---|---|
author | Yau-Qiu, Zhi Xin Picó, Catalina Rodríguez, Ana María Palou, Andreu |
author_facet | Yau-Qiu, Zhi Xin Picó, Catalina Rodríguez, Ana María Palou, Andreu |
author_sort | Yau-Qiu, Zhi Xin |
collection | PubMed |
description | Prenatal leptin is key to regulating foetal growth and early metabolic programming. The presence of intact leptin in rat foetal (at late gestation) and neonatal (immediately after birth) stomach content and mucosa has been previously described, suggesting that it may act as a regulatory nutrient for the neonate rats, be internalised by the stomach, and play a physiological role early in life, which requires to be further investigated, including its origin. We aimed to study the ontogeny of the presence of leptin in the foetal stomach and key extraembryonic tissues in rats at late gestation (days 18–21). Leptin concentration was determined by enzyme-linked immunosorbent assay, and placental leptin immunolocalisation was analysed by immunohistochemistry. Leptin showed a sudden appearance in the amniotic fluid (AF) at day 20 of gestation, gastric content (swallowed AF), stomach, and umbilical cord, significantly increasing at day 21. Leptin levels in these fluids and tissues were positively correlated. In the placenta, leptin was detectable at all the studied days, but its localisation changed from widespread throughout the placenta at day 18 to well-defined in the labyrinth zone from day 19 onwards. The results support a possible internalisation of AF leptin by the immature stomach of near-term foetuses and suggest that changes in placental leptin localisation might help to explain the sudden appearance of leptin in AF at gestational day 20, with potential physiological significance regarding short-term feeding control and metabolic programming in the developing offspring. |
format | Online Article Text |
id | pubmed-7551401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75514012020-10-14 Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin Yau-Qiu, Zhi Xin Picó, Catalina Rodríguez, Ana María Palou, Andreu Nutrients Article Prenatal leptin is key to regulating foetal growth and early metabolic programming. The presence of intact leptin in rat foetal (at late gestation) and neonatal (immediately after birth) stomach content and mucosa has been previously described, suggesting that it may act as a regulatory nutrient for the neonate rats, be internalised by the stomach, and play a physiological role early in life, which requires to be further investigated, including its origin. We aimed to study the ontogeny of the presence of leptin in the foetal stomach and key extraembryonic tissues in rats at late gestation (days 18–21). Leptin concentration was determined by enzyme-linked immunosorbent assay, and placental leptin immunolocalisation was analysed by immunohistochemistry. Leptin showed a sudden appearance in the amniotic fluid (AF) at day 20 of gestation, gastric content (swallowed AF), stomach, and umbilical cord, significantly increasing at day 21. Leptin levels in these fluids and tissues were positively correlated. In the placenta, leptin was detectable at all the studied days, but its localisation changed from widespread throughout the placenta at day 18 to well-defined in the labyrinth zone from day 19 onwards. The results support a possible internalisation of AF leptin by the immature stomach of near-term foetuses and suggest that changes in placental leptin localisation might help to explain the sudden appearance of leptin in AF at gestational day 20, with potential physiological significance regarding short-term feeding control and metabolic programming in the developing offspring. MDPI 2020-08-21 /pmc/articles/PMC7551401/ /pubmed/32825787 http://dx.doi.org/10.3390/nu12092542 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yau-Qiu, Zhi Xin Picó, Catalina Rodríguez, Ana María Palou, Andreu Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title | Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title_full | Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title_fullStr | Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title_full_unstemmed | Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title_short | Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin |
title_sort | leptin distribution in rat foetal and extraembryonic tissues in late gestation: a physiological view of amniotic fluid leptin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551401/ https://www.ncbi.nlm.nih.gov/pubmed/32825787 http://dx.doi.org/10.3390/nu12092542 |
work_keys_str_mv | AT yauqiuzhixin leptindistributioninratfoetalandextraembryonictissuesinlategestationaphysiologicalviewofamnioticfluidleptin AT picocatalina leptindistributioninratfoetalandextraembryonictissuesinlategestationaphysiologicalviewofamnioticfluidleptin AT rodriguezanamaria leptindistributioninratfoetalandextraembryonictissuesinlategestationaphysiologicalviewofamnioticfluidleptin AT palouandreu leptindistributioninratfoetalandextraembryonictissuesinlategestationaphysiologicalviewofamnioticfluidleptin |