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Impact of Storage Conditions on the Breast Milk Peptidome

Human donor milk (HDM) provides appropriate nutrition and offers protective functions in preterm infants. The aim of the study is to examine the impact of different storage conditions on the stability of the human breast milk peptidome. HDM was directly frozen at −80 °C or stored at −20 °C (120 h),...

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Autores principales: Howland, Vanessa, Klaedtke, Maik, Ruhnau, Johanna, Dhople, Vishnu M., Grabe, Hans J., Völker, Uwe, Heckmann, Matthias, Hammer, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551425/
https://www.ncbi.nlm.nih.gov/pubmed/32911625
http://dx.doi.org/10.3390/nu12092733
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author Howland, Vanessa
Klaedtke, Maik
Ruhnau, Johanna
Dhople, Vishnu M.
Grabe, Hans J.
Völker, Uwe
Heckmann, Matthias
Hammer, Elke
author_facet Howland, Vanessa
Klaedtke, Maik
Ruhnau, Johanna
Dhople, Vishnu M.
Grabe, Hans J.
Völker, Uwe
Heckmann, Matthias
Hammer, Elke
author_sort Howland, Vanessa
collection PubMed
description Human donor milk (HDM) provides appropriate nutrition and offers protective functions in preterm infants. The aim of the study is to examine the impact of different storage conditions on the stability of the human breast milk peptidome. HDM was directly frozen at −80 °C or stored at −20 °C (120 h), 4 °C (6 h), or room temperature (RT for 6 or 24 h). The milk peptidome was profiled by mass spectrometry after peptide collection by ultrafiltration. Profiling of the peptidome covered 3587 peptides corresponding to 212 proteins. The variance of the peptidome increased with storage temperature and time and varied for different peptides. The highest impact was observed when samples were stored at RT. Smaller but significant effects were still observed in samples stored at 4 °C, while samples showed highest similarity to those immediately frozen at −80 °C when stored at −20 °C. Peptide structures after storage at RT for 24 h point to the increased activity of thrombin and other proteases cleaving proteins at lysine/arginine. The results point to an ongoing protein degradation/peptide production by milk-derived proteases. They underline the need for immediate freezing of HDM at −20 °C or −80 °C to prevent degradation of peptides and enable reproducible investigation of prospectively collected samples.
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spelling pubmed-75514252020-10-14 Impact of Storage Conditions on the Breast Milk Peptidome Howland, Vanessa Klaedtke, Maik Ruhnau, Johanna Dhople, Vishnu M. Grabe, Hans J. Völker, Uwe Heckmann, Matthias Hammer, Elke Nutrients Article Human donor milk (HDM) provides appropriate nutrition and offers protective functions in preterm infants. The aim of the study is to examine the impact of different storage conditions on the stability of the human breast milk peptidome. HDM was directly frozen at −80 °C or stored at −20 °C (120 h), 4 °C (6 h), or room temperature (RT for 6 or 24 h). The milk peptidome was profiled by mass spectrometry after peptide collection by ultrafiltration. Profiling of the peptidome covered 3587 peptides corresponding to 212 proteins. The variance of the peptidome increased with storage temperature and time and varied for different peptides. The highest impact was observed when samples were stored at RT. Smaller but significant effects were still observed in samples stored at 4 °C, while samples showed highest similarity to those immediately frozen at −80 °C when stored at −20 °C. Peptide structures after storage at RT for 24 h point to the increased activity of thrombin and other proteases cleaving proteins at lysine/arginine. The results point to an ongoing protein degradation/peptide production by milk-derived proteases. They underline the need for immediate freezing of HDM at −20 °C or −80 °C to prevent degradation of peptides and enable reproducible investigation of prospectively collected samples. MDPI 2020-09-08 /pmc/articles/PMC7551425/ /pubmed/32911625 http://dx.doi.org/10.3390/nu12092733 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Howland, Vanessa
Klaedtke, Maik
Ruhnau, Johanna
Dhople, Vishnu M.
Grabe, Hans J.
Völker, Uwe
Heckmann, Matthias
Hammer, Elke
Impact of Storage Conditions on the Breast Milk Peptidome
title Impact of Storage Conditions on the Breast Milk Peptidome
title_full Impact of Storage Conditions on the Breast Milk Peptidome
title_fullStr Impact of Storage Conditions on the Breast Milk Peptidome
title_full_unstemmed Impact of Storage Conditions on the Breast Milk Peptidome
title_short Impact of Storage Conditions on the Breast Milk Peptidome
title_sort impact of storage conditions on the breast milk peptidome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551425/
https://www.ncbi.nlm.nih.gov/pubmed/32911625
http://dx.doi.org/10.3390/nu12092733
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