Cargando…

The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits

Deoxynivalenol (DON) is a mycotoxin found in grains that poses a potential threat to human and animal health, and the gastrointestinal tract is the primary target organ. There are few studies focused on the toxicology of DON to rabbits, especially on the relation among DON, microbiota, and the gut-a...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Chunyang, Huang, Libo, Wang, Pengwei, Liu, Quancheng, Wang, Jinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551620/
https://www.ncbi.nlm.nih.gov/pubmed/32899719
http://dx.doi.org/10.3390/toxins12090569
_version_ 1783593222181748736
author Wang, Chunyang
Huang, Libo
Wang, Pengwei
Liu, Quancheng
Wang, Jinquan
author_facet Wang, Chunyang
Huang, Libo
Wang, Pengwei
Liu, Quancheng
Wang, Jinquan
author_sort Wang, Chunyang
collection PubMed
description Deoxynivalenol (DON) is a mycotoxin found in grains that poses a potential threat to human and animal health, and the gastrointestinal tract is the primary target organ. There are few studies focused on the toxicology of DON to rabbits, especially on the relation among DON, microbiota, and the gut-associated lymphoid tissue. A total of 30 weaned rabbits (35 d) were evenly divided into the control group and DON group (1.5 mg/kg bodyweight (BW)) based on their body weight. After a 24-day trial, the ultrastructures of the sacculus rotundus and vermiform appendix were observed using a scanning electron microscope and transmission electron microscopy. The morphology and microflora in the ileum, caecum, and colon were also examined. The results proved that the ultrastructure of the sacculus rotundus and vermiform appendix, as well as the integrity of the intestinal barrier (especially for the ileum), were impaired after DON was administrated to the rabbits. Compared to the control group, the relative abundance and diversity of the microflora decreased in all three intestinal segments in the DON group, particularly in the ileum and caecum. In conclusion, the toxic effect of DON on weaned rabbits may be performed by destroying the structure of the sacculus rotundus and vermiform appendix, as well as affecting the structure and diversity of the intestinal flora.
format Online
Article
Text
id pubmed-7551620
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75516202020-10-14 The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits Wang, Chunyang Huang, Libo Wang, Pengwei Liu, Quancheng Wang, Jinquan Toxins (Basel) Article Deoxynivalenol (DON) is a mycotoxin found in grains that poses a potential threat to human and animal health, and the gastrointestinal tract is the primary target organ. There are few studies focused on the toxicology of DON to rabbits, especially on the relation among DON, microbiota, and the gut-associated lymphoid tissue. A total of 30 weaned rabbits (35 d) were evenly divided into the control group and DON group (1.5 mg/kg bodyweight (BW)) based on their body weight. After a 24-day trial, the ultrastructures of the sacculus rotundus and vermiform appendix were observed using a scanning electron microscope and transmission electron microscopy. The morphology and microflora in the ileum, caecum, and colon were also examined. The results proved that the ultrastructure of the sacculus rotundus and vermiform appendix, as well as the integrity of the intestinal barrier (especially for the ileum), were impaired after DON was administrated to the rabbits. Compared to the control group, the relative abundance and diversity of the microflora decreased in all three intestinal segments in the DON group, particularly in the ileum and caecum. In conclusion, the toxic effect of DON on weaned rabbits may be performed by destroying the structure of the sacculus rotundus and vermiform appendix, as well as affecting the structure and diversity of the intestinal flora. MDPI 2020-09-04 /pmc/articles/PMC7551620/ /pubmed/32899719 http://dx.doi.org/10.3390/toxins12090569 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Chunyang
Huang, Libo
Wang, Pengwei
Liu, Quancheng
Wang, Jinquan
The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title_full The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title_fullStr The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title_full_unstemmed The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title_short The Effects of Deoxynivalenol on the Ultrastructure of the Sacculus Rotundus and Vermiform Appendix, as Well as the Intestinal Microbiota of Weaned Rabbits
title_sort effects of deoxynivalenol on the ultrastructure of the sacculus rotundus and vermiform appendix, as well as the intestinal microbiota of weaned rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551620/
https://www.ncbi.nlm.nih.gov/pubmed/32899719
http://dx.doi.org/10.3390/toxins12090569
work_keys_str_mv AT wangchunyang theeffectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT huanglibo theeffectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT wangpengwei theeffectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT liuquancheng theeffectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT wangjinquan theeffectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT wangchunyang effectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT huanglibo effectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT wangpengwei effectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT liuquancheng effectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits
AT wangjinquan effectsofdeoxynivalenolontheultrastructureofthesacculusrotundusandvermiformappendixaswellastheintestinalmicrobiotaofweanedrabbits