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Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity

The anti-obesity effects of RL (a 3:1 mixture of Panax ginseng saponin fractions and Glycyrrhiza glabra L. extracts) on 3T3-L1 adipocytes and C57BL/6J obese mice were evaluated at different concentrations. We investigated the anti-obesity effects of RL through lipid accumulation inhibition rate, ser...

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Autores principales: Zheng, Yulong, Lee, Eun-Hye, Lee, Ji-Hyun, In, Gyo, Kim, JongHan, Lee, Mi-Hyang, Lee, Ok-Hwan, Kang, Il-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551630/
https://www.ncbi.nlm.nih.gov/pubmed/32916854
http://dx.doi.org/10.3390/nu12092744
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author Zheng, Yulong
Lee, Eun-Hye
Lee, Ji-Hyun
In, Gyo
Kim, JongHan
Lee, Mi-Hyang
Lee, Ok-Hwan
Kang, Il-Jun
author_facet Zheng, Yulong
Lee, Eun-Hye
Lee, Ji-Hyun
In, Gyo
Kim, JongHan
Lee, Mi-Hyang
Lee, Ok-Hwan
Kang, Il-Jun
author_sort Zheng, Yulong
collection PubMed
description The anti-obesity effects of RL (a 3:1 mixture of Panax ginseng saponin fractions and Glycyrrhiza glabra L. extracts) on 3T3-L1 adipocytes and C57BL/6J obese mice were evaluated at different concentrations. We investigated the anti-obesity effects of RL through lipid accumulation inhibition rate, serum lipid composition analysis, adipose tissue size, adipogenic transcription factors and AMPK pathway. RL inhibited the lipid accumulation of 3T3-L1 adipocytes in a dose-dependent manner at concentrations of 50–200 μg/mL without cytotoxicity (50–400 μg/mL). Oral administration of RL at the highest concentration (400 mg/kg/day) did not cause significant liver toxicity in high-fat diet-induced obese mice. RL stimulated adiponectin secretion in a dose-dependent manner and primarily mediates the AMPK pathway to inhibit triglyceride synthesis and attenuate adipocyte hypertrophy. RL significantly reduced weight in obese mice, but none of the body weight, adipose tissue weight, serum triglyceride level, and AMPK pathway activation degree showed any difference between dosing concentrations of 200 and 400 mg/kg/day. Therefore, 200 mg/kg/day of RL is the optimal preclinical concentration, which can be a reference concentration for conversion into a human clinical trial dose.
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spelling pubmed-75516302020-10-14 Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity Zheng, Yulong Lee, Eun-Hye Lee, Ji-Hyun In, Gyo Kim, JongHan Lee, Mi-Hyang Lee, Ok-Hwan Kang, Il-Jun Nutrients Article The anti-obesity effects of RL (a 3:1 mixture of Panax ginseng saponin fractions and Glycyrrhiza glabra L. extracts) on 3T3-L1 adipocytes and C57BL/6J obese mice were evaluated at different concentrations. We investigated the anti-obesity effects of RL through lipid accumulation inhibition rate, serum lipid composition analysis, adipose tissue size, adipogenic transcription factors and AMPK pathway. RL inhibited the lipid accumulation of 3T3-L1 adipocytes in a dose-dependent manner at concentrations of 50–200 μg/mL without cytotoxicity (50–400 μg/mL). Oral administration of RL at the highest concentration (400 mg/kg/day) did not cause significant liver toxicity in high-fat diet-induced obese mice. RL stimulated adiponectin secretion in a dose-dependent manner and primarily mediates the AMPK pathway to inhibit triglyceride synthesis and attenuate adipocyte hypertrophy. RL significantly reduced weight in obese mice, but none of the body weight, adipose tissue weight, serum triglyceride level, and AMPK pathway activation degree showed any difference between dosing concentrations of 200 and 400 mg/kg/day. Therefore, 200 mg/kg/day of RL is the optimal preclinical concentration, which can be a reference concentration for conversion into a human clinical trial dose. MDPI 2020-09-09 /pmc/articles/PMC7551630/ /pubmed/32916854 http://dx.doi.org/10.3390/nu12092744 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Yulong
Lee, Eun-Hye
Lee, Ji-Hyun
In, Gyo
Kim, JongHan
Lee, Mi-Hyang
Lee, Ok-Hwan
Kang, Il-Jun
Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title_full Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title_fullStr Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title_full_unstemmed Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title_short Preclinical Research on a Mixture of Red Ginseng and Licorice Extracts in the Treatment and Prevention of Obesity
title_sort preclinical research on a mixture of red ginseng and licorice extracts in the treatment and prevention of obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551630/
https://www.ncbi.nlm.nih.gov/pubmed/32916854
http://dx.doi.org/10.3390/nu12092744
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