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Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience
Saporin, which is extracted from Saponaria officinalis, is a protein toxin that inactivates ribosomes. Saporin itself is non-selective toxin but acquires high specificity after conjugation with different ligands such as signaling peptides or antibodies to some surface proteins expressed in a chosen...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551693/ https://www.ncbi.nlm.nih.gov/pubmed/32854372 http://dx.doi.org/10.3390/toxins12090546 |
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author | Bolshakov, Alexey P. Stepanichev, Mikhail Yu. Dobryakova, Yulia V. Spivak, Yulia S. Markevich, Vladimir A. |
author_facet | Bolshakov, Alexey P. Stepanichev, Mikhail Yu. Dobryakova, Yulia V. Spivak, Yulia S. Markevich, Vladimir A. |
author_sort | Bolshakov, Alexey P. |
collection | PubMed |
description | Saporin, which is extracted from Saponaria officinalis, is a protein toxin that inactivates ribosomes. Saporin itself is non-selective toxin but acquires high specificity after conjugation with different ligands such as signaling peptides or antibodies to some surface proteins expressed in a chosen cell subpopulation. The saporin-based conjugated toxins were widely adopted in neuroscience as a convenient tool to induce highly selective degeneration of desired cell subpopulation. Induction of selective cell death is one of approaches used to model neurodegenerative diseases, study functions of certain cell subpopulations in the brain, and therapy. Here, we review studies where saporin-based conjugates were used to analyze cell mechanisms of sleep, general anesthesia, epilepsy, pain, and development of Parkinson’s and Alzheimer’s diseases. Limitations and future perspectives of use of saporin-based toxins in neuroscience are discussed. |
format | Online Article Text |
id | pubmed-7551693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75516932020-10-14 Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience Bolshakov, Alexey P. Stepanichev, Mikhail Yu. Dobryakova, Yulia V. Spivak, Yulia S. Markevich, Vladimir A. Toxins (Basel) Review Saporin, which is extracted from Saponaria officinalis, is a protein toxin that inactivates ribosomes. Saporin itself is non-selective toxin but acquires high specificity after conjugation with different ligands such as signaling peptides or antibodies to some surface proteins expressed in a chosen cell subpopulation. The saporin-based conjugated toxins were widely adopted in neuroscience as a convenient tool to induce highly selective degeneration of desired cell subpopulation. Induction of selective cell death is one of approaches used to model neurodegenerative diseases, study functions of certain cell subpopulations in the brain, and therapy. Here, we review studies where saporin-based conjugates were used to analyze cell mechanisms of sleep, general anesthesia, epilepsy, pain, and development of Parkinson’s and Alzheimer’s diseases. Limitations and future perspectives of use of saporin-based toxins in neuroscience are discussed. MDPI 2020-08-25 /pmc/articles/PMC7551693/ /pubmed/32854372 http://dx.doi.org/10.3390/toxins12090546 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Bolshakov, Alexey P. Stepanichev, Mikhail Yu. Dobryakova, Yulia V. Spivak, Yulia S. Markevich, Vladimir A. Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title | Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title_full | Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title_fullStr | Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title_full_unstemmed | Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title_short | Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience |
title_sort | saporin from saponaria officinalis as a tool for experimental research, modeling, and therapy in neuroscience |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551693/ https://www.ncbi.nlm.nih.gov/pubmed/32854372 http://dx.doi.org/10.3390/toxins12090546 |
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