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Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker

CD63, a member of the tetraspanin superfamily, is used as a marker of late endosomes and lysosome-related organelles, as well as a marker of exosomes. Here, we selected rare isotype variants of TS63 by sorting hybridoma cells on the basis of their high expression of surface immunoglobulins of the Ig...

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Detalles Bibliográficos
Autores principales: Charrin, Stéphanie, Palmulli, Roberta, Billard, Martine, Clay, Denis, Boucheix, Claude, Van Niel, Guillaume, Rubinstein, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551895/
https://www.ncbi.nlm.nih.gov/pubmed/32630723
http://dx.doi.org/10.3390/antib9030029