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Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker
CD63, a member of the tetraspanin superfamily, is used as a marker of late endosomes and lysosome-related organelles, as well as a marker of exosomes. Here, we selected rare isotype variants of TS63 by sorting hybridoma cells on the basis of their high expression of surface immunoglobulins of the Ig...
Autores principales: | Charrin, Stéphanie, Palmulli, Roberta, Billard, Martine, Clay, Denis, Boucheix, Claude, Van Niel, Guillaume, Rubinstein, Eric |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551895/ https://www.ncbi.nlm.nih.gov/pubmed/32630723 http://dx.doi.org/10.3390/antib9030029 |
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