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Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s

The rabies virus strain Komatsugawa (Koma), which was isolated from a dog in Tokyo in the 1940s before eradication of rabies in Japan in 1957, is known as the only existent Japanese field strain (street strain). Although this strain potentially provides a useful model to study rabies pathogenesis, l...

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Autores principales: Takahashi, Tatsuki, Inukai, Maho, Sasaki, Michihito, Potratz, Madlin, Jarusombuti, Supasiri, Fujii, Yuji, Nishiyama, Shoko, Finke, Stefan, Yamada, Kentaro, Sakai, Hiroki, Sawa, Hirofumi, Nishizono, Akira, Sugiyama, Makoto, Ito, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552007/
https://www.ncbi.nlm.nih.gov/pubmed/32825306
http://dx.doi.org/10.3390/v12090914
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author Takahashi, Tatsuki
Inukai, Maho
Sasaki, Michihito
Potratz, Madlin
Jarusombuti, Supasiri
Fujii, Yuji
Nishiyama, Shoko
Finke, Stefan
Yamada, Kentaro
Sakai, Hiroki
Sawa, Hirofumi
Nishizono, Akira
Sugiyama, Makoto
Ito, Naoto
author_facet Takahashi, Tatsuki
Inukai, Maho
Sasaki, Michihito
Potratz, Madlin
Jarusombuti, Supasiri
Fujii, Yuji
Nishiyama, Shoko
Finke, Stefan
Yamada, Kentaro
Sakai, Hiroki
Sawa, Hirofumi
Nishizono, Akira
Sugiyama, Makoto
Ito, Naoto
author_sort Takahashi, Tatsuki
collection PubMed
description The rabies virus strain Komatsugawa (Koma), which was isolated from a dog in Tokyo in the 1940s before eradication of rabies in Japan in 1957, is known as the only existent Japanese field strain (street strain). Although this strain potentially provides a useful model to study rabies pathogenesis, little is known about its genetic and phenotypic properties. Notably, this strain underwent serial passages in rodents after isolation, indicating the possibility that it may have lost biological characteristics as a street strain. In this study, to evaluate the utility of the Koma strain for studying rabies pathogenesis, we examined the genetic properties and in vitro and in vivo phenotypes. Genome-wide genetic analyses showed that, consistent with previous findings from partial sequence analyses, the Koma strain is closely related to a Russian street strain within the Arctic-related phylogenetic clade. Phenotypic examinations in vitro revealed that the Koma strain and the representative street strains are less neurotropic than the laboratory strains. Examination by using a mouse model demonstrated that the Koma strain and the street strains are more neuroinvasive than the laboratory strains. These findings indicate that the Koma strain retains phenotypes similar to those of street strains, and is therefore useful for studying rabies pathogenesis.
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spelling pubmed-75520072020-10-14 Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s Takahashi, Tatsuki Inukai, Maho Sasaki, Michihito Potratz, Madlin Jarusombuti, Supasiri Fujii, Yuji Nishiyama, Shoko Finke, Stefan Yamada, Kentaro Sakai, Hiroki Sawa, Hirofumi Nishizono, Akira Sugiyama, Makoto Ito, Naoto Viruses Article The rabies virus strain Komatsugawa (Koma), which was isolated from a dog in Tokyo in the 1940s before eradication of rabies in Japan in 1957, is known as the only existent Japanese field strain (street strain). Although this strain potentially provides a useful model to study rabies pathogenesis, little is known about its genetic and phenotypic properties. Notably, this strain underwent serial passages in rodents after isolation, indicating the possibility that it may have lost biological characteristics as a street strain. In this study, to evaluate the utility of the Koma strain for studying rabies pathogenesis, we examined the genetic properties and in vitro and in vivo phenotypes. Genome-wide genetic analyses showed that, consistent with previous findings from partial sequence analyses, the Koma strain is closely related to a Russian street strain within the Arctic-related phylogenetic clade. Phenotypic examinations in vitro revealed that the Koma strain and the representative street strains are less neurotropic than the laboratory strains. Examination by using a mouse model demonstrated that the Koma strain and the street strains are more neuroinvasive than the laboratory strains. These findings indicate that the Koma strain retains phenotypes similar to those of street strains, and is therefore useful for studying rabies pathogenesis. MDPI 2020-08-20 /pmc/articles/PMC7552007/ /pubmed/32825306 http://dx.doi.org/10.3390/v12090914 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takahashi, Tatsuki
Inukai, Maho
Sasaki, Michihito
Potratz, Madlin
Jarusombuti, Supasiri
Fujii, Yuji
Nishiyama, Shoko
Finke, Stefan
Yamada, Kentaro
Sakai, Hiroki
Sawa, Hirofumi
Nishizono, Akira
Sugiyama, Makoto
Ito, Naoto
Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title_full Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title_fullStr Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title_full_unstemmed Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title_short Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s
title_sort genetic and phenotypic characterization of a rabies virus strain isolated from a dog in tokyo, japan in the 1940s
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552007/
https://www.ncbi.nlm.nih.gov/pubmed/32825306
http://dx.doi.org/10.3390/v12090914
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