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Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2
Cyprinid herpesvirus 2 (CyHV-2), which infects goldfish and crucian carp causing high mortality, is an emerging viral pathogen worldwide. The genome of CyHV-2 is large and comprises double-stranded DNA, including several genes similar to cyprinid herpesvirus 1, ictalurid herpesvirus-1, cyprinid herp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552009/ https://www.ncbi.nlm.nih.gov/pubmed/32906668 http://dx.doi.org/10.3390/v12090994 |
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author | Tang, Ruizhe Lu, Liqun Wang, Beiyang Yu, Jiao Wang, Hao |
author_facet | Tang, Ruizhe Lu, Liqun Wang, Beiyang Yu, Jiao Wang, Hao |
author_sort | Tang, Ruizhe |
collection | PubMed |
description | Cyprinid herpesvirus 2 (CyHV-2), which infects goldfish and crucian carp causing high mortality, is an emerging viral pathogen worldwide. The genome of CyHV-2 is large and comprises double-stranded DNA, including several genes similar to cyprinid herpesvirus 1, ictalurid herpesvirus-1, cyprinid herpesvirus 3, and ranid herpesvirus-1. Genes of DNA viruses are expressed in three temporal phases: immediate-early (IE), early (E), and late (L) genes. Viral IE genes initiate transcription as soon as the virus enters the host, without viral DNA replication. IE gene products enable the efficient expression of E and L genes or regulate the host to initiate virus replication. In the present study, five IE genes of CyHV-2 were identified, including open reading frame (ORF)54, ORF121, ORF141, ORF147, and ORF155. Time course analysis and reverse transcription polymerase chain reaction confirmed five IE genes, thirty-four E genes, and thirty-nine L genes. In addition, all 150 ORFs identified in the CyHV-2 genome are transcribed, and are expressed in chronological order, similar to other herpesviruses. This study is the first to identify the IE genes of CyHV-2, which will provide more information for viral molecular characterization. |
format | Online Article Text |
id | pubmed-7552009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75520092020-10-14 Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 Tang, Ruizhe Lu, Liqun Wang, Beiyang Yu, Jiao Wang, Hao Viruses Article Cyprinid herpesvirus 2 (CyHV-2), which infects goldfish and crucian carp causing high mortality, is an emerging viral pathogen worldwide. The genome of CyHV-2 is large and comprises double-stranded DNA, including several genes similar to cyprinid herpesvirus 1, ictalurid herpesvirus-1, cyprinid herpesvirus 3, and ranid herpesvirus-1. Genes of DNA viruses are expressed in three temporal phases: immediate-early (IE), early (E), and late (L) genes. Viral IE genes initiate transcription as soon as the virus enters the host, without viral DNA replication. IE gene products enable the efficient expression of E and L genes or regulate the host to initiate virus replication. In the present study, five IE genes of CyHV-2 were identified, including open reading frame (ORF)54, ORF121, ORF141, ORF147, and ORF155. Time course analysis and reverse transcription polymerase chain reaction confirmed five IE genes, thirty-four E genes, and thirty-nine L genes. In addition, all 150 ORFs identified in the CyHV-2 genome are transcribed, and are expressed in chronological order, similar to other herpesviruses. This study is the first to identify the IE genes of CyHV-2, which will provide more information for viral molecular characterization. MDPI 2020-09-07 /pmc/articles/PMC7552009/ /pubmed/32906668 http://dx.doi.org/10.3390/v12090994 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Ruizhe Lu, Liqun Wang, Beiyang Yu, Jiao Wang, Hao Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title | Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title_full | Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title_fullStr | Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title_full_unstemmed | Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title_short | Identification of the Immediate-Early Genes of Cyprinid Herpesvirus 2 |
title_sort | identification of the immediate-early genes of cyprinid herpesvirus 2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552009/ https://www.ncbi.nlm.nih.gov/pubmed/32906668 http://dx.doi.org/10.3390/v12090994 |
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