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A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes
Hepatitis B virus (HBV) is a para-retrovirus that reverse transcribes its pregenomic RNA into relaxed circular DNA inside viral nucleocapsids. The number of HBV genomes produced in vitro is typically quantified using commercial silica-membrane-based nucleic acid purification kits to isolate total DN...
Autores principales: | Jo, Eunji, Yang, Jaewon, Koenig, Alexander, Yoon, Seung Kew, Windisch, Marc P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552047/ https://www.ncbi.nlm.nih.gov/pubmed/32846983 http://dx.doi.org/10.3390/v12090928 |
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