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Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland
A metagenomic study was performed on 498 female and 40 male Aedes albopictus mosquitos collected in August and September 2019 in Ticino, a region in southern Switzerland, to address the question regarding the risk of the local transmission of zoonotic viruses. A total of 13 viruses from seven differ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552062/ https://www.ncbi.nlm.nih.gov/pubmed/32846980 http://dx.doi.org/10.3390/v12090929 |
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author | Kubacki, Jakub Flacio, Eleonora Qi, Weihong Guidi, Valeria Tonolla, Mauro Fraefel, Cornel |
author_facet | Kubacki, Jakub Flacio, Eleonora Qi, Weihong Guidi, Valeria Tonolla, Mauro Fraefel, Cornel |
author_sort | Kubacki, Jakub |
collection | PubMed |
description | A metagenomic study was performed on 498 female and 40 male Aedes albopictus mosquitos collected in August and September 2019 in Ticino, a region in southern Switzerland, to address the question regarding the risk of the local transmission of zoonotic viruses. A total of 13 viruses from seven different virus families and several unclassified viral taxa were identified. Reads of insect-specific flaviviruses were present in all pools, and a complete genome of aedes flavivirus was assembled and phylogenetically analysed. The most abundant virus was Wenzhou sobemo-like virus, assembled from 1.3 × 10(5) to 3.6 × 10(6) reads in each pool. In a pool of male mosquitos, a complete genome of aedes Iflavi-like virus was detected and phylogenetically analysed. Most importantly, genomes of human pathogenic viruses were not found. This is the first study to determine the virome of Ae. albopictus from Switzerland and forms a baseline for future longitudinal investigations concerning the potential role of Ae. albopictus as a vector of clinically relevant viruses. |
format | Online Article Text |
id | pubmed-7552062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75520622020-10-14 Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland Kubacki, Jakub Flacio, Eleonora Qi, Weihong Guidi, Valeria Tonolla, Mauro Fraefel, Cornel Viruses Article A metagenomic study was performed on 498 female and 40 male Aedes albopictus mosquitos collected in August and September 2019 in Ticino, a region in southern Switzerland, to address the question regarding the risk of the local transmission of zoonotic viruses. A total of 13 viruses from seven different virus families and several unclassified viral taxa were identified. Reads of insect-specific flaviviruses were present in all pools, and a complete genome of aedes flavivirus was assembled and phylogenetically analysed. The most abundant virus was Wenzhou sobemo-like virus, assembled from 1.3 × 10(5) to 3.6 × 10(6) reads in each pool. In a pool of male mosquitos, a complete genome of aedes Iflavi-like virus was detected and phylogenetically analysed. Most importantly, genomes of human pathogenic viruses were not found. This is the first study to determine the virome of Ae. albopictus from Switzerland and forms a baseline for future longitudinal investigations concerning the potential role of Ae. albopictus as a vector of clinically relevant viruses. MDPI 2020-08-24 /pmc/articles/PMC7552062/ /pubmed/32846980 http://dx.doi.org/10.3390/v12090929 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kubacki, Jakub Flacio, Eleonora Qi, Weihong Guidi, Valeria Tonolla, Mauro Fraefel, Cornel Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title | Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title_full | Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title_fullStr | Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title_full_unstemmed | Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title_short | Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland |
title_sort | viral metagenomic analysis of aedes albopictus mosquitos from southern switzerland |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552062/ https://www.ncbi.nlm.nih.gov/pubmed/32846980 http://dx.doi.org/10.3390/v12090929 |
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