Mucosal Hallmarks in the Alimentary Canal of Northern Pike Esox lucius (Linnaeus)

SIMPLE SUMMARY: In vertebrates, mucous cells are one of the main cellular components of the gut mucosal system, which secrete different mucin types involved in several functions. Endocrine cells are scattered in the epithelium of the gut mucosa, and they produce and release regulatory molecules affecting food intake and nutrition. The goal of this study was to obtain data on quantitative distribution of mucous and endocrine cell types in the alimentary canal of the northern pike (Esox lucius), using histochemistry and immunofluorescence. In the stomach of pike, there is a high abundance of mixed mucins, with the acid component contributing to the lubrication of mucosae, where they are associated with the rapid passage of digesta through the intestine. Neutral mucins increase in the intestine aborally. The distribution of endocrine cells of the diffuse endocrine system shows the presence of somatostatin and catecholamine-secreting endocrine cells and the lack of gastrin-secreting endocrine cells. We show a close regulatory relation between endocrine and mucous cells of the gut mucosal system involved in the physiology of fish nutrition. Results confirmed the relationship between the carnivorous diet and the gut mucins distribution of northern pike; indeed, our data provide very important information to ichthyologists who study dietary behavior of species. ABSTRACT: On the basis of trophic behavior, fish are classified as herbivores, carnivores, omnivores, or detritivores. Epithelial mucous cells secrete mucin types specific to diet and digestive function. Mucus secretion is regulated mainly by molecular modulators produced by epithelial endocrine cells in response to luminal or tissue stimuli. These modulators are involved in control of food intake and digestive functions. Immunohistochemical and immunofluorescence studies were conducted on 10 adult northern pike (Esox lucius Linnaeus, 1758) from Lake Piediluco (Central Italy) to quantify distribution of sub-types of mucous and endocrine cells in alimentary mucosal epithelium. Neutral mucins predominated in the esophagus, and mixed and acidic mucins predominated in stomach and intestine. The gastric epithelium contained endocrine cells secreting somatostatin, tyrosine hydroxylase, and substance P. Mucous cells secreting neutral mucins increased in number from proximal to distal intestine, with endocrine cells containing substance P in the proximal intestine and those containing Leu-enkephalin throughout the intestine. Lectin histochemistry of gut sections revealed an abundance of N-acetyl-glucosamine and N-acetyl-galactosamine as carbohydrate residues on the mucin chain. The quantity and content of endocrine and mucous cells in the alimentary canal of E. lucius showed a direct relationship with its diet.