The Inclusion of the Microalga Scenedesmus sp. in Diets for Rainbow Trout, Onchorhynchus mykiss, Juveniles

SIMPLE SUMMARY: This study aimed to evaluate the suitability of the microalga Scenedesmus sp. in diets for rainbow trout juveniles. Considering previous results with similar species, the authors tested the inclusion of this microalga at 5% in diets (48% protein and 18% lipid levels). After 45 days, neither trout growth nor feed efficiency parameters nor fillet proximate composition were negatively affected by the inclusion of the microalga in the diet. In addition, provision of the diet containing the microalga did not lead to observable negative effects on liver or intestinal histological organization and function. Dietary Scenedesmus sp. improved the nutritional quality of the fillet in terms of n-3 polyunsaturated fatty acid (PUFA) levels, especially docosahexanoic acid (DHA), although it did alter the color of the fillet. In addition, feeding rainbow trout with diets containing Scenedesmus sp. modified the lipid class composition in the liver by increasing the levels of polar phospholipids with regard to triacylglycerides; results that may be attributed to dietary-induced changes in lipid metabolism. Results showed that the green microalga Scenedesmus sp. is a safe ingredient for compound feeds in rainbow trout when considering fish growth performance, condition, and health parameters, although the visual appearance of the fillet was affected. ABSTRACT: A nutritional study was conducted to evaluate the inclusion of the green microalga Scenedesmus sp. at 5% (SCE-5) as an alternative fishmeal ingredient. This microalga was tested with four replicates during 45 days using isolipidic (18%), isoproteic (48%), and isoenergetic (1.9 MJ kg(−1)) diets. Fish fed Scenedesmus sp. showed similar growth and feed efficiency parameters as the control group. Regarding the digestive function, the SCE-5 diet enhanced the activity of alkaline pancreatic proteases, whereas it did not affect that of intestinal enzymes involved in nutrient absorption. No histological alterations were found in fish fed the SCE-5 diet, although a higher density of goblet cells in the anterior intestine and changes in gut microbiome diversity were found in this group, which collectively suggests positive effects of this green microalga on the intestine. Dietary Scenedesmus sp. improved the fillet’s nutritional quality in terms of n-3 polyunsaturated fatty acid (PUFA) levels, although it also increased its yellowish color. The overall results of this study showed that Scenedesmus sp. is a safe ingredient for compound feeds in rainbow trout when considering fish growth performance, animal condition, and health parameters, although it substantially affected the color of the fillet that may potentially affect consumers’ preferences.