Bacterial Load of the Teat Apex Skin and Associated Factors at Herd Level

SIMPLE SUMMARY: Bacterial load on the teat apex of dairy cows that causes intramammary infections is to a large extent due to environmental impacts. The aim of our study was to describe factors at herd level that are associated with bacterial load of environmental mastitis pathogens on the teat end’s skin. On visits to 31 dairy farms over a one-year period, farm conditions were documented, and environmental bacterial loads were examined. We found seasonal fluctuations and direct correlations between the temperature–humidity index (THI) in the barn and the bacterial load at the teat end. Significantly more environmental mastitis pathogens were found in herds with a high percentage of normal and slightly rough teat ends. The time since the last fresh bedding was added to the cubicles, as well as the frequency in which cubicles were cleaned, also affect the pathogen load on the teat skin. Pre-cleaning teats before milking as well as post-dipping after milking showed a decreasing effect of teat-skin bacterial load at the herd level. ABSTRACT: In order to reduce antimicrobial treatment and prevent environmental mastitis, the aim of the present study was to investigate associations between herd level factors and microbial load on teat ends with environmental mastitis pathogens. Quarterly farm visits of 31 dairy farms over a one-year period were used for statistical analysis. During each farm visit, teat-skin swabs, bedding and air samples were taken and management practices and herd parameters were documented. Total mesophilic bacteria, esculin-positive streptococci and coliform bacteria were examined in the laboratory procedures from teat skin and environmental samples. Esculin-positive streptococci and coliform bacteria on teat ends increased with high temperature–humidity indices (THI) in the barn during the spring and summer. Significantly more coliform bacteria on teat ends were found in herds with an increased percentage of normal or slightly rough teat ends. Cleaning cubicles more frequently, pre-cleaning teats before milking as well as post-dipping them after milking had a decreasing effect of teat-skin load with total mesophilic and coliform bacteria at the herd level. To conclude, teat-skin bacterial load with environmental pathogens is subject to fluctuations and can be influenced by aspects of farm hygiene.