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Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells
OBJECTIVE: The mosquito transmitted RNA virus dengue virus (DENV) shows significant variation as a consequence of the lack of proofreading activity of the RNA-dependent RNA polymerase that synthesizes new virus genomes. How this variation affects DENV replication, and how this in turn impacts drug d...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552352/ https://www.ncbi.nlm.nih.gov/pubmed/33046135 http://dx.doi.org/10.1186/s13104-020-05325-6 |
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author | Hitakarun, Atitaya Ramphan, Suwipa Wikan, Nitwara Smith, Duncan R. |
author_facet | Hitakarun, Atitaya Ramphan, Suwipa Wikan, Nitwara Smith, Duncan R. |
author_sort | Hitakarun, Atitaya |
collection | PubMed |
description | OBJECTIVE: The mosquito transmitted RNA virus dengue virus (DENV) shows significant variation as a consequence of the lack of proofreading activity of the RNA-dependent RNA polymerase that synthesizes new virus genomes. How this variation affects DENV replication, and how this in turn impacts drug development remains largely unknown. Given the technical limitations in working with large numbers of isolates few studies have sought to investigate this area. This study used a panel of 14 DENV isolates of different serotypes and origins to determine how much virus replication in Aedes albopictus C6/36 cells was affected by DENV variability. RESULTS: The results showed that there was considerable variation, with peak titers ranging from 6Log10 to 8Log10, and maximum titer being reached from day 3 to day 9 post infection. While strains from DENV 1 and 4 serotypes showed considerable uniformity, DENV 2 and 3 strains showed much greater variation. Overall, these results show that serotype specific strain variation can have a significant impact on DENV replication, suggesting that studies either investigating DENV pathogenesis or developing drug therapeutics should consider the contribution of DENV variability. |
format | Online Article Text |
id | pubmed-7552352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75523522020-10-13 Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells Hitakarun, Atitaya Ramphan, Suwipa Wikan, Nitwara Smith, Duncan R. BMC Res Notes Research Note OBJECTIVE: The mosquito transmitted RNA virus dengue virus (DENV) shows significant variation as a consequence of the lack of proofreading activity of the RNA-dependent RNA polymerase that synthesizes new virus genomes. How this variation affects DENV replication, and how this in turn impacts drug development remains largely unknown. Given the technical limitations in working with large numbers of isolates few studies have sought to investigate this area. This study used a panel of 14 DENV isolates of different serotypes and origins to determine how much virus replication in Aedes albopictus C6/36 cells was affected by DENV variability. RESULTS: The results showed that there was considerable variation, with peak titers ranging from 6Log10 to 8Log10, and maximum titer being reached from day 3 to day 9 post infection. While strains from DENV 1 and 4 serotypes showed considerable uniformity, DENV 2 and 3 strains showed much greater variation. Overall, these results show that serotype specific strain variation can have a significant impact on DENV replication, suggesting that studies either investigating DENV pathogenesis or developing drug therapeutics should consider the contribution of DENV variability. BioMed Central 2020-10-12 /pmc/articles/PMC7552352/ /pubmed/33046135 http://dx.doi.org/10.1186/s13104-020-05325-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Hitakarun, Atitaya Ramphan, Suwipa Wikan, Nitwara Smith, Duncan R. Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title | Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title_full | Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title_fullStr | Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title_full_unstemmed | Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title_short | Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells |
title_sort | analysis of the virus propagation profile of 14 dengue virus isolates in aedes albopictus c6/36 cells |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552352/ https://www.ncbi.nlm.nih.gov/pubmed/33046135 http://dx.doi.org/10.1186/s13104-020-05325-6 |
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