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Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection

Porcine reproductive and respiratory syndrome virus (PRRSV) infection is the most important viral disease causing severe economic losses in the swine industry. However, mechanisms underlying gene expression control in immunity-responsible tissues at different time points during PRRSV infection are p...

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Autores principales: Lim, Byeonghwi, Kim, Sangwook, Lim, Kyu-Sang, Jeong, Chang-Gi, Kim, Seung-Chai, Lee, Sang-Myeong, Park, Choi-Kyu, te Pas, Marinus F. W., Gho, Haesu, Kim, Tae-Hun, Lee, Kyung-Tai, Kim, Won-Il, Kim, Jun-Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552595/
https://www.ncbi.nlm.nih.gov/pubmed/33050948
http://dx.doi.org/10.1186/s13567-020-00850-5
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author Lim, Byeonghwi
Kim, Sangwook
Lim, Kyu-Sang
Jeong, Chang-Gi
Kim, Seung-Chai
Lee, Sang-Myeong
Park, Choi-Kyu
te Pas, Marinus F. W.
Gho, Haesu
Kim, Tae-Hun
Lee, Kyung-Tai
Kim, Won-Il
Kim, Jun-Mo
author_facet Lim, Byeonghwi
Kim, Sangwook
Lim, Kyu-Sang
Jeong, Chang-Gi
Kim, Seung-Chai
Lee, Sang-Myeong
Park, Choi-Kyu
te Pas, Marinus F. W.
Gho, Haesu
Kim, Tae-Hun
Lee, Kyung-Tai
Kim, Won-Il
Kim, Jun-Mo
author_sort Lim, Byeonghwi
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) infection is the most important viral disease causing severe economic losses in the swine industry. However, mechanisms underlying gene expression control in immunity-responsible tissues at different time points during PRRSV infection are poorly understood. We constructed an integrated gene co-expression network and identified tissue- and time-dependent biological mechanisms of PRRSV infection through bioinformatics analysis using three tissues (lungs, bronchial lymph nodes [BLNs], and tonsils) via RNA-Seq. Three groups with specific expression patterns (i.e., the 3-dpi, lung, and BLN groups) were discovered. The 3 dpi-specific group showed antiviral and innate-immune signalling similar to the case for influenza A infection. Moreover, we observed adaptive immune responses in the lung-specific group based on various cytokines, while the BLN-specific group showed down-regulated AMPK signalling related to viral replication. Our study may provide comprehensive insights into PRRSV infection, as well as useful information for vaccine development.
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spelling pubmed-75525952020-10-14 Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection Lim, Byeonghwi Kim, Sangwook Lim, Kyu-Sang Jeong, Chang-Gi Kim, Seung-Chai Lee, Sang-Myeong Park, Choi-Kyu te Pas, Marinus F. W. Gho, Haesu Kim, Tae-Hun Lee, Kyung-Tai Kim, Won-Il Kim, Jun-Mo Vet Res Research Article Porcine reproductive and respiratory syndrome virus (PRRSV) infection is the most important viral disease causing severe economic losses in the swine industry. However, mechanisms underlying gene expression control in immunity-responsible tissues at different time points during PRRSV infection are poorly understood. We constructed an integrated gene co-expression network and identified tissue- and time-dependent biological mechanisms of PRRSV infection through bioinformatics analysis using three tissues (lungs, bronchial lymph nodes [BLNs], and tonsils) via RNA-Seq. Three groups with specific expression patterns (i.e., the 3-dpi, lung, and BLN groups) were discovered. The 3 dpi-specific group showed antiviral and innate-immune signalling similar to the case for influenza A infection. Moreover, we observed adaptive immune responses in the lung-specific group based on various cytokines, while the BLN-specific group showed down-regulated AMPK signalling related to viral replication. Our study may provide comprehensive insights into PRRSV infection, as well as useful information for vaccine development. BioMed Central 2020-10-13 2020 /pmc/articles/PMC7552595/ /pubmed/33050948 http://dx.doi.org/10.1186/s13567-020-00850-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Lim, Byeonghwi
Kim, Sangwook
Lim, Kyu-Sang
Jeong, Chang-Gi
Kim, Seung-Chai
Lee, Sang-Myeong
Park, Choi-Kyu
te Pas, Marinus F. W.
Gho, Haesu
Kim, Tae-Hun
Lee, Kyung-Tai
Kim, Won-Il
Kim, Jun-Mo
Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title_full Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title_fullStr Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title_full_unstemmed Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title_short Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection
title_sort integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to prrsv infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552595/
https://www.ncbi.nlm.nih.gov/pubmed/33050948
http://dx.doi.org/10.1186/s13567-020-00850-5
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