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Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia

Cryptosporidium is a major etiological agent of diarrhoeal diseases among children and immune-compromised individuals in sub-Saharan African countries. We conducted a study to determine the prevalence and genetic characteristics of Cryptosporidium spp. in stool samples from patients with diarrhoea w...

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Autores principales: Mulunda, Namwiinga Rozaria, Hayashida, Kyoko, Yamagishi, Junya, Sianongo, Sandie, Munsaka, Gilbert, Sugimoto, Chihiro, Mutengo, Mable Mwale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553232/
https://www.ncbi.nlm.nih.gov/pubmed/33048665
http://dx.doi.org/10.1051/parasite/2020050
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author Mulunda, Namwiinga Rozaria
Hayashida, Kyoko
Yamagishi, Junya
Sianongo, Sandie
Munsaka, Gilbert
Sugimoto, Chihiro
Mutengo, Mable Mwale
author_facet Mulunda, Namwiinga Rozaria
Hayashida, Kyoko
Yamagishi, Junya
Sianongo, Sandie
Munsaka, Gilbert
Sugimoto, Chihiro
Mutengo, Mable Mwale
author_sort Mulunda, Namwiinga Rozaria
collection PubMed
description Cryptosporidium is a major etiological agent of diarrhoeal diseases among children and immune-compromised individuals in sub-Saharan African countries. We conducted a study to determine the prevalence and genetic characteristics of Cryptosporidium spp. in stool samples from patients with diarrhoea who presented at the University Teaching Hospital in Lusaka, Zambia. Cryptosporidium species and subtypes from 71 microscopically confirmed cryptosporidiosis stool samples collected between 2017 and 2019 were determined by polymerase chain reaction followed by partial sequencing of the small subunit rRNA and 60-kDa glycoprotein (gp60) gene. Additionally, data for the period between 2014 and 2019 were reviewed and analysed for cryptosporidiosis seasonal and age distribution. Cryptosporidium was more prevalent in the rainy season. The highest number of cases was reported among the 1–4 year age group. By sequence analysis of the 71 positive isolates, Cryptosporidium hominis (n = 42; 59.2%), C. parvum (n = 27; 38%), C. felis (n = 1; 1.4%), and C. meleagridis (n = 1; 1.4%) were identified. Four C. hominis subtype families (Ia, Ib, Id, and Ie) and three C. parvum subtype families (IIc, IIe, and IIs) were identified. The most frequent subtypes were IeA11G3T3 (n = 20; 28.2%), IIcA5G3 (n = 12; 16.9%), IIeA12G1 (n = 11; 15.5%) and IaA30R3 (n = 10; 14.1%). The observed species/subtypes of C. hominis and C. parvum indicated that the infection was mainly transmitted through the anthroponotic route. The identification of C. felis and C. meleagridis suggests that an atypical zoonotic transmission cycle also exists.
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spelling pubmed-75532322020-10-20 Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia Mulunda, Namwiinga Rozaria Hayashida, Kyoko Yamagishi, Junya Sianongo, Sandie Munsaka, Gilbert Sugimoto, Chihiro Mutengo, Mable Mwale Parasite Research Article Cryptosporidium is a major etiological agent of diarrhoeal diseases among children and immune-compromised individuals in sub-Saharan African countries. We conducted a study to determine the prevalence and genetic characteristics of Cryptosporidium spp. in stool samples from patients with diarrhoea who presented at the University Teaching Hospital in Lusaka, Zambia. Cryptosporidium species and subtypes from 71 microscopically confirmed cryptosporidiosis stool samples collected between 2017 and 2019 were determined by polymerase chain reaction followed by partial sequencing of the small subunit rRNA and 60-kDa glycoprotein (gp60) gene. Additionally, data for the period between 2014 and 2019 were reviewed and analysed for cryptosporidiosis seasonal and age distribution. Cryptosporidium was more prevalent in the rainy season. The highest number of cases was reported among the 1–4 year age group. By sequence analysis of the 71 positive isolates, Cryptosporidium hominis (n = 42; 59.2%), C. parvum (n = 27; 38%), C. felis (n = 1; 1.4%), and C. meleagridis (n = 1; 1.4%) were identified. Four C. hominis subtype families (Ia, Ib, Id, and Ie) and three C. parvum subtype families (IIc, IIe, and IIs) were identified. The most frequent subtypes were IeA11G3T3 (n = 20; 28.2%), IIcA5G3 (n = 12; 16.9%), IIeA12G1 (n = 11; 15.5%) and IaA30R3 (n = 10; 14.1%). The observed species/subtypes of C. hominis and C. parvum indicated that the infection was mainly transmitted through the anthroponotic route. The identification of C. felis and C. meleagridis suggests that an atypical zoonotic transmission cycle also exists. EDP Sciences 2020-10-13 /pmc/articles/PMC7553232/ /pubmed/33048665 http://dx.doi.org/10.1051/parasite/2020050 Text en © N.R. Mulunda et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mulunda, Namwiinga Rozaria
Hayashida, Kyoko
Yamagishi, Junya
Sianongo, Sandie
Munsaka, Gilbert
Sugimoto, Chihiro
Mutengo, Mable Mwale
Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title_full Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title_fullStr Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title_full_unstemmed Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title_short Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia
title_sort molecular characterization of cryptosporidium spp. from patients with diarrhoea in lusaka, zambia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553232/
https://www.ncbi.nlm.nih.gov/pubmed/33048665
http://dx.doi.org/10.1051/parasite/2020050
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