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NOD2 receptor is crucial for protecting against the digestive form of Chagas disease

Digestive and cardiodigestive forms of Chagas’ disease are observed in 2% to 27% of the patients, depending on their geographic location, Trypanosoma cruzi strain and immunopathological responses. The aim of this work was to evaluate the role of NOD2 innate immune receptor in the pathogenesis of the...

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Autores principales: Pereira, Nathalie de Sena, Queiroga, Tamyres Bernadete Dantas, da Silva, Denis Dantas, Nascimento, Manuela Sales Lima, de Andrade, Cléber Mesquita, de Souto, Janeusa Trindade, Ricci, Mayra Fernanda, Arantes, Rosa Maria Esteves, Zamboni, Dario Simões, Chiari, Egler, da Câmara, Antônia Cláudia Jácome, Galvão, Lúcia Maria da Cunha, Guedes, Paulo Marcos Matta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553797/
https://www.ncbi.nlm.nih.gov/pubmed/32986710
http://dx.doi.org/10.1371/journal.pntd.0008667
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author Pereira, Nathalie de Sena
Queiroga, Tamyres Bernadete Dantas
da Silva, Denis Dantas
Nascimento, Manuela Sales Lima
de Andrade, Cléber Mesquita
de Souto, Janeusa Trindade
Ricci, Mayra Fernanda
Arantes, Rosa Maria Esteves
Zamboni, Dario Simões
Chiari, Egler
da Câmara, Antônia Cláudia Jácome
Galvão, Lúcia Maria da Cunha
Guedes, Paulo Marcos Matta
author_facet Pereira, Nathalie de Sena
Queiroga, Tamyres Bernadete Dantas
da Silva, Denis Dantas
Nascimento, Manuela Sales Lima
de Andrade, Cléber Mesquita
de Souto, Janeusa Trindade
Ricci, Mayra Fernanda
Arantes, Rosa Maria Esteves
Zamboni, Dario Simões
Chiari, Egler
da Câmara, Antônia Cláudia Jácome
Galvão, Lúcia Maria da Cunha
Guedes, Paulo Marcos Matta
author_sort Pereira, Nathalie de Sena
collection PubMed
description Digestive and cardiodigestive forms of Chagas’ disease are observed in 2% to 27% of the patients, depending on their geographic location, Trypanosoma cruzi strain and immunopathological responses. The aim of this work was to evaluate the role of NOD2 innate immune receptor in the pathogenesis of the digestive system in Chagas’ disease. Patients with digestive form of the disease showed lower mRNA expression of NOD2, higher expression of RIP2 and α-defensin 6, compared to indeterminate form, detected by Real-time PCR in peripheral blood mononuclear cells. In addition, there was a negative correlation between the expression of NOD2 and the degree of dilation of the esophagus, sigmoid and rectum in those patients. The infection of NOD2(-/-) mice with T. cruzi strain isolated from the digestive patient induced a decrease in intestinal motility. Histopathological analysis of the colon and jejunum of NOD2(-/-) and wild type C57BL/6 animals revealed discrete inflammatory foci during the acute phase of infection. Interestingly, during the chronic phase of the infection there was inflammation and hypertrophy of the longitudinal and circular muscular layer more pronounced in the colon and jejunum from NOD2(-/-) animals, when compared to wild type C57BL/6 mice. Together, our results suggest that NOD2 plays a protective role against the development of digestive form of Chagas’ disease.
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spelling pubmed-75537972020-10-21 NOD2 receptor is crucial for protecting against the digestive form of Chagas disease Pereira, Nathalie de Sena Queiroga, Tamyres Bernadete Dantas da Silva, Denis Dantas Nascimento, Manuela Sales Lima de Andrade, Cléber Mesquita de Souto, Janeusa Trindade Ricci, Mayra Fernanda Arantes, Rosa Maria Esteves Zamboni, Dario Simões Chiari, Egler da Câmara, Antônia Cláudia Jácome Galvão, Lúcia Maria da Cunha Guedes, Paulo Marcos Matta PLoS Negl Trop Dis Research Article Digestive and cardiodigestive forms of Chagas’ disease are observed in 2% to 27% of the patients, depending on their geographic location, Trypanosoma cruzi strain and immunopathological responses. The aim of this work was to evaluate the role of NOD2 innate immune receptor in the pathogenesis of the digestive system in Chagas’ disease. Patients with digestive form of the disease showed lower mRNA expression of NOD2, higher expression of RIP2 and α-defensin 6, compared to indeterminate form, detected by Real-time PCR in peripheral blood mononuclear cells. In addition, there was a negative correlation between the expression of NOD2 and the degree of dilation of the esophagus, sigmoid and rectum in those patients. The infection of NOD2(-/-) mice with T. cruzi strain isolated from the digestive patient induced a decrease in intestinal motility. Histopathological analysis of the colon and jejunum of NOD2(-/-) and wild type C57BL/6 animals revealed discrete inflammatory foci during the acute phase of infection. Interestingly, during the chronic phase of the infection there was inflammation and hypertrophy of the longitudinal and circular muscular layer more pronounced in the colon and jejunum from NOD2(-/-) animals, when compared to wild type C57BL/6 mice. Together, our results suggest that NOD2 plays a protective role against the development of digestive form of Chagas’ disease. Public Library of Science 2020-09-28 /pmc/articles/PMC7553797/ /pubmed/32986710 http://dx.doi.org/10.1371/journal.pntd.0008667 Text en © 2020 Pereira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pereira, Nathalie de Sena
Queiroga, Tamyres Bernadete Dantas
da Silva, Denis Dantas
Nascimento, Manuela Sales Lima
de Andrade, Cléber Mesquita
de Souto, Janeusa Trindade
Ricci, Mayra Fernanda
Arantes, Rosa Maria Esteves
Zamboni, Dario Simões
Chiari, Egler
da Câmara, Antônia Cláudia Jácome
Galvão, Lúcia Maria da Cunha
Guedes, Paulo Marcos Matta
NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title_full NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title_fullStr NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title_full_unstemmed NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title_short NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
title_sort nod2 receptor is crucial for protecting against the digestive form of chagas disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553797/
https://www.ncbi.nlm.nih.gov/pubmed/32986710
http://dx.doi.org/10.1371/journal.pntd.0008667
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