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Protein-crystal detection with a compact multimodal multiphoton microscope
There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, m...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553921/ https://www.ncbi.nlm.nih.gov/pubmed/33051587 http://dx.doi.org/10.1038/s42003-020-01275-8 |
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author | Cheng, Qing-di Chung, Hsiang-Yu Schubert, Robin Chia, Shih-Hsuan Falke, Sven Mudogo, Celestin Nzanzu Kärtner, Franz X. Chang, Guoqing Betzel, Christian |
author_facet | Cheng, Qing-di Chung, Hsiang-Yu Schubert, Robin Chia, Shih-Hsuan Falke, Sven Mudogo, Celestin Nzanzu Kärtner, Franz X. Chang, Guoqing Betzel, Christian |
author_sort | Cheng, Qing-di |
collection | PubMed |
description | There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future. |
format | Online Article Text |
id | pubmed-7553921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75539212020-10-19 Protein-crystal detection with a compact multimodal multiphoton microscope Cheng, Qing-di Chung, Hsiang-Yu Schubert, Robin Chia, Shih-Hsuan Falke, Sven Mudogo, Celestin Nzanzu Kärtner, Franz X. Chang, Guoqing Betzel, Christian Commun Biol Article There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future. Nature Publishing Group UK 2020-10-13 /pmc/articles/PMC7553921/ /pubmed/33051587 http://dx.doi.org/10.1038/s42003-020-01275-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Cheng, Qing-di Chung, Hsiang-Yu Schubert, Robin Chia, Shih-Hsuan Falke, Sven Mudogo, Celestin Nzanzu Kärtner, Franz X. Chang, Guoqing Betzel, Christian Protein-crystal detection with a compact multimodal multiphoton microscope |
title | Protein-crystal detection with a compact multimodal multiphoton microscope |
title_full | Protein-crystal detection with a compact multimodal multiphoton microscope |
title_fullStr | Protein-crystal detection with a compact multimodal multiphoton microscope |
title_full_unstemmed | Protein-crystal detection with a compact multimodal multiphoton microscope |
title_short | Protein-crystal detection with a compact multimodal multiphoton microscope |
title_sort | protein-crystal detection with a compact multimodal multiphoton microscope |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553921/ https://www.ncbi.nlm.nih.gov/pubmed/33051587 http://dx.doi.org/10.1038/s42003-020-01275-8 |
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