Characterization of thymine microcrystals by CARS and SHG microscopy

Identification of chemically homologous microcrystals in a polycrystal sample is a big challenge and requires developing specific highly sensitive tools. Second harmonic (SHG) and coherent anti-Stokes Raman scattering (CARS) spectroscopy can be used to reveal arrangement of thymine molecules, one of...

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Autores principales: Dementjev, Andrej, Rutkauskas, Danielis, Polovy, Ivan, Macernis, Mindaugas, Abramavicius, Darius, Valkunas, Leonas, Dovbeshko, Galina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553945/
https://www.ncbi.nlm.nih.gov/pubmed/33051591
http://dx.doi.org/10.1038/s41598-020-74305-4
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author Dementjev, Andrej
Rutkauskas, Danielis
Polovy, Ivan
Macernis, Mindaugas
Abramavicius, Darius
Valkunas, Leonas
Dovbeshko, Galina
author_facet Dementjev, Andrej
Rutkauskas, Danielis
Polovy, Ivan
Macernis, Mindaugas
Abramavicius, Darius
Valkunas, Leonas
Dovbeshko, Galina
author_sort Dementjev, Andrej
collection PubMed
description Identification of chemically homologous microcrystals in a polycrystal sample is a big challenge and requires developing specific highly sensitive tools. Second harmonic (SHG) and coherent anti-Stokes Raman scattering (CARS) spectroscopy can be used to reveal arrangement of thymine molecules, one of the DNA bases, in microcrystalline sample. Strong dependence of CARS and SHG intensity on the orientation of the linear polarization of the excitation light allows to obtain high resolution images of thymine microcrystals by additionally utilizing the scanning microscopy technique. Experimental findings and theoretical interpretation of the results are compared. Presented experimental data together with quantum chemistry-based theoretical interpretation allowed us to determine the most probable organization of the thymine molecules.
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spelling pubmed-75539452020-10-14 Characterization of thymine microcrystals by CARS and SHG microscopy Dementjev, Andrej Rutkauskas, Danielis Polovy, Ivan Macernis, Mindaugas Abramavicius, Darius Valkunas, Leonas Dovbeshko, Galina Sci Rep Article Identification of chemically homologous microcrystals in a polycrystal sample is a big challenge and requires developing specific highly sensitive tools. Second harmonic (SHG) and coherent anti-Stokes Raman scattering (CARS) spectroscopy can be used to reveal arrangement of thymine molecules, one of the DNA bases, in microcrystalline sample. Strong dependence of CARS and SHG intensity on the orientation of the linear polarization of the excitation light allows to obtain high resolution images of thymine microcrystals by additionally utilizing the scanning microscopy technique. Experimental findings and theoretical interpretation of the results are compared. Presented experimental data together with quantum chemistry-based theoretical interpretation allowed us to determine the most probable organization of the thymine molecules. Nature Publishing Group UK 2020-10-13 /pmc/articles/PMC7553945/ /pubmed/33051591 http://dx.doi.org/10.1038/s41598-020-74305-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dementjev, Andrej
Rutkauskas, Danielis
Polovy, Ivan
Macernis, Mindaugas
Abramavicius, Darius
Valkunas, Leonas
Dovbeshko, Galina
Characterization of thymine microcrystals by CARS and SHG microscopy
title Characterization of thymine microcrystals by CARS and SHG microscopy
title_full Characterization of thymine microcrystals by CARS and SHG microscopy
title_fullStr Characterization of thymine microcrystals by CARS and SHG microscopy
title_full_unstemmed Characterization of thymine microcrystals by CARS and SHG microscopy
title_short Characterization of thymine microcrystals by CARS and SHG microscopy
title_sort characterization of thymine microcrystals by cars and shg microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553945/
https://www.ncbi.nlm.nih.gov/pubmed/33051591
http://dx.doi.org/10.1038/s41598-020-74305-4
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