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High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates

BACKGROUND: Efflux pumps are involved in resistance of Acinetobacter baumannii isolates to antimicrobial agents. AdeABC efflux pump is one of the RND superfamily efflux pump and consists of adeA (membrane fusion), adeB (multidrug transporter) and adeC (outer membrane) genes. In this study, the frequ...

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Autores principales: RANJBAR, Reza, ZAYERI, Shahin, AFSHAR, Davoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554404/
https://www.ncbi.nlm.nih.gov/pubmed/33083331
http://dx.doi.org/10.18502/ijph.v49i8.3898
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author RANJBAR, Reza
ZAYERI, Shahin
AFSHAR, Davoud
author_facet RANJBAR, Reza
ZAYERI, Shahin
AFSHAR, Davoud
author_sort RANJBAR, Reza
collection PubMed
description BACKGROUND: Efflux pumps are involved in resistance of Acinetobacter baumannii isolates to antimicrobial agents. AdeABC efflux pump is one of the RND superfamily efflux pump and consists of adeA (membrane fusion), adeB (multidrug transporter) and adeC (outer membrane) genes. In this study, the frequency of adeA, adeB and adeC genes among A. baumannii isolates with resistance to erythromycin, trimethoprim, meropenem and imipenem was investigated. METHODS: Overall, 79 strains of A. baumannii were isolated from patients admitted to two major hospitals in Tehran during 2016. Antibiotic susceptibility testing was determined by disc diffusion and microdilution methods according to Clinical and Laboratory Standards Institute (CLSI) guideline. The presence of adeA, adeB and adeC genes was also determined using Multiplex PCR assay. RESULTS: The highest and the lowest resistance among A. baumannii isolates were to trimethoprim (93%) and erythromycin (53%), respectively. The frequency of adeA, adeB and adeC genes was 96.2%, 96.2% and 91.1 % respectively. There was a significant relationship between imipenem resistance and presence of efflux pump genes (P<0.05). CONCLUSION: According to the high prevalence of the AdeABC efflux system genes, it may involve in resistance of clinical isolates of A. baumannii to imipenem, especially.
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spelling pubmed-75544042020-10-19 High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates RANJBAR, Reza ZAYERI, Shahin AFSHAR, Davoud Iran J Public Health Original Article BACKGROUND: Efflux pumps are involved in resistance of Acinetobacter baumannii isolates to antimicrobial agents. AdeABC efflux pump is one of the RND superfamily efflux pump and consists of adeA (membrane fusion), adeB (multidrug transporter) and adeC (outer membrane) genes. In this study, the frequency of adeA, adeB and adeC genes among A. baumannii isolates with resistance to erythromycin, trimethoprim, meropenem and imipenem was investigated. METHODS: Overall, 79 strains of A. baumannii were isolated from patients admitted to two major hospitals in Tehran during 2016. Antibiotic susceptibility testing was determined by disc diffusion and microdilution methods according to Clinical and Laboratory Standards Institute (CLSI) guideline. The presence of adeA, adeB and adeC genes was also determined using Multiplex PCR assay. RESULTS: The highest and the lowest resistance among A. baumannii isolates were to trimethoprim (93%) and erythromycin (53%), respectively. The frequency of adeA, adeB and adeC genes was 96.2%, 96.2% and 91.1 % respectively. There was a significant relationship between imipenem resistance and presence of efflux pump genes (P<0.05). CONCLUSION: According to the high prevalence of the AdeABC efflux system genes, it may involve in resistance of clinical isolates of A. baumannii to imipenem, especially. Tehran University of Medical Sciences 2020-08 /pmc/articles/PMC7554404/ /pubmed/33083331 http://dx.doi.org/10.18502/ijph.v49i8.3898 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
RANJBAR, Reza
ZAYERI, Shahin
AFSHAR, Davoud
High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title_full High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title_fullStr High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title_full_unstemmed High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title_short High Frequency of AdeA, AdeB and AdeC Genes among Acinetobacter baumannii Isolates
title_sort high frequency of adea, adeb and adec genes among acinetobacter baumannii isolates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554404/
https://www.ncbi.nlm.nih.gov/pubmed/33083331
http://dx.doi.org/10.18502/ijph.v49i8.3898
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