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A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice

Pulmonary infections caused by Mycobacterium abscessus (MA) have increased over recent decades, affecting individuals with underlying pathologies such as chronic obstructive pulmonary disease, bronchiectasis and, especially, cystic fibrosis. The lack of a representative and standardized model of chr...

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Autores principales: Riva, Camilla, Tortoli, Enrico, Cugnata, Federica, Sanvito, Francesca, Esposito, Antonio, Rossi, Marco, Colarieti, Anna, Canu, Tamara, Cigana, Cristina, Bragonzi, Alessandra, Loré, Nicola Ivan, Miotto, Paolo, Cirillo, Daniela Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554715/
https://www.ncbi.nlm.nih.gov/pubmed/32916885
http://dx.doi.org/10.3390/ijms21186590
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author Riva, Camilla
Tortoli, Enrico
Cugnata, Federica
Sanvito, Francesca
Esposito, Antonio
Rossi, Marco
Colarieti, Anna
Canu, Tamara
Cigana, Cristina
Bragonzi, Alessandra
Loré, Nicola Ivan
Miotto, Paolo
Cirillo, Daniela Maria
author_facet Riva, Camilla
Tortoli, Enrico
Cugnata, Federica
Sanvito, Francesca
Esposito, Antonio
Rossi, Marco
Colarieti, Anna
Canu, Tamara
Cigana, Cristina
Bragonzi, Alessandra
Loré, Nicola Ivan
Miotto, Paolo
Cirillo, Daniela Maria
author_sort Riva, Camilla
collection PubMed
description Pulmonary infections caused by Mycobacterium abscessus (MA) have increased over recent decades, affecting individuals with underlying pathologies such as chronic obstructive pulmonary disease, bronchiectasis and, especially, cystic fibrosis. The lack of a representative and standardized model of chronic infection in mice has limited steps forward in the field of MA pulmonary infection. To overcome this challenge, we refined the method of agar beads to establish MA chronic infection in immunocompetent mice. We evaluated bacterial count, lung pathology and markers of inflammation and we performed longitudinal studies with magnetic resonance imaging (MRI) up to three months after MA infection. In this model, MA was able to establish a persistent lung infection for up to two months and with minimal systemic spread. Lung histopathological analysis revealed granulomatous inflammation around bronchi characterized by the presence of lymphocytes, aggregates of vacuolated histiocytes and a few neutrophils, mimicking the damage observed in humans. Furthermore, MA lung lesions were successfully monitored for the first time by MRI. The availability of this murine model and the introduction of the successfully longitudinal monitoring of the murine lung lesions with MRI pave the way for further investigations on the impact of MA pathogenesis and the efficacy of novel treatments.
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spelling pubmed-75547152020-10-19 A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice Riva, Camilla Tortoli, Enrico Cugnata, Federica Sanvito, Francesca Esposito, Antonio Rossi, Marco Colarieti, Anna Canu, Tamara Cigana, Cristina Bragonzi, Alessandra Loré, Nicola Ivan Miotto, Paolo Cirillo, Daniela Maria Int J Mol Sci Article Pulmonary infections caused by Mycobacterium abscessus (MA) have increased over recent decades, affecting individuals with underlying pathologies such as chronic obstructive pulmonary disease, bronchiectasis and, especially, cystic fibrosis. The lack of a representative and standardized model of chronic infection in mice has limited steps forward in the field of MA pulmonary infection. To overcome this challenge, we refined the method of agar beads to establish MA chronic infection in immunocompetent mice. We evaluated bacterial count, lung pathology and markers of inflammation and we performed longitudinal studies with magnetic resonance imaging (MRI) up to three months after MA infection. In this model, MA was able to establish a persistent lung infection for up to two months and with minimal systemic spread. Lung histopathological analysis revealed granulomatous inflammation around bronchi characterized by the presence of lymphocytes, aggregates of vacuolated histiocytes and a few neutrophils, mimicking the damage observed in humans. Furthermore, MA lung lesions were successfully monitored for the first time by MRI. The availability of this murine model and the introduction of the successfully longitudinal monitoring of the murine lung lesions with MRI pave the way for further investigations on the impact of MA pathogenesis and the efficacy of novel treatments. MDPI 2020-09-09 /pmc/articles/PMC7554715/ /pubmed/32916885 http://dx.doi.org/10.3390/ijms21186590 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Riva, Camilla
Tortoli, Enrico
Cugnata, Federica
Sanvito, Francesca
Esposito, Antonio
Rossi, Marco
Colarieti, Anna
Canu, Tamara
Cigana, Cristina
Bragonzi, Alessandra
Loré, Nicola Ivan
Miotto, Paolo
Cirillo, Daniela Maria
A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title_full A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title_fullStr A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title_full_unstemmed A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title_short A New Model of Chronic Mycobacterium abscessus Lung Infection in Immunocompetent Mice
title_sort new model of chronic mycobacterium abscessus lung infection in immunocompetent mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554715/
https://www.ncbi.nlm.nih.gov/pubmed/32916885
http://dx.doi.org/10.3390/ijms21186590
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