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Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice
Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA base repair and reducing activity. However, the role of APE1/Ref-1 in atherosclerosis is unclear. Herein, we investigated the role of APE1/Ref-1 in atherosclerotic apolipoprotein E (ApoE(−/−)) mice fed with a Western...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555038/ https://www.ncbi.nlm.nih.gov/pubmed/32967121 http://dx.doi.org/10.3390/biomedicines8090366 |
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author | Lee, Yu Ran Joo, Hee Kyoung Lee, Eun-Ok Park, Myoung Soo Cho, Hyun Sil Kim, Sungmin Jin, Hao Jeong, Jin-Ok Kim, Cuk-Seong Jeon, Byeong Hwa |
author_facet | Lee, Yu Ran Joo, Hee Kyoung Lee, Eun-Ok Park, Myoung Soo Cho, Hyun Sil Kim, Sungmin Jin, Hao Jeong, Jin-Ok Kim, Cuk-Seong Jeon, Byeong Hwa |
author_sort | Lee, Yu Ran |
collection | PubMed |
description | Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA base repair and reducing activity. However, the role of APE1/Ref-1 in atherosclerosis is unclear. Herein, we investigated the role of APE1/Ref-1 in atherosclerotic apolipoprotein E (ApoE(−/−)) mice fed with a Western-type diet. We found that serologic APE1/Ref-1 was strongly correlated with vascular inflammation in these mice. Neutrophil/lymphocyte ratio (NLR), endothelial cell/macrophage activation, and atherosclerotic plaque formation, reflected by atherosclerotic inflammation, were increased in the ApoE(−/−) mice fed with a Western-type diet. APE1/Ref-1 expression was upregulated in aortic tissues of these mice, and was co-localized with cells positive for cluster of differentiation 31 (CD31) and galectin-3, suggesting endothelial cell/macrophage expression of APE1/Ref-1. Interestingly, APE1/Ref-1 plasma levels of ApoE(−/−) mice fed with a Western-type diet were significantly increased compared with those of the mice fed with normal diet (15.76 ± 3.19 ng/mL vs. 3.51 ± 0.50 ng/mL, p < 0.05), and were suppressed by atorvastatin administration. Correlation analysis showed high correlation between plasma APE1/Ref-1 levels and NLR, a marker of systemic inflammation. The cut-off value for APE1/Ref-1 for predicting atherosclerotic inflammation at 4.903 ng/mL showed sensitivity of 100% and specificity of 91%. We conclude that APE1/Ref-1 expression is upregulated in aortic endothelial cells/macrophages of atherosclerotic mice, and that plasma APE1/Ref-1 levels could predict atherosclerotic inflammation. |
format | Online Article Text |
id | pubmed-7555038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75550382020-10-14 Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice Lee, Yu Ran Joo, Hee Kyoung Lee, Eun-Ok Park, Myoung Soo Cho, Hyun Sil Kim, Sungmin Jin, Hao Jeong, Jin-Ok Kim, Cuk-Seong Jeon, Byeong Hwa Biomedicines Article Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA base repair and reducing activity. However, the role of APE1/Ref-1 in atherosclerosis is unclear. Herein, we investigated the role of APE1/Ref-1 in atherosclerotic apolipoprotein E (ApoE(−/−)) mice fed with a Western-type diet. We found that serologic APE1/Ref-1 was strongly correlated with vascular inflammation in these mice. Neutrophil/lymphocyte ratio (NLR), endothelial cell/macrophage activation, and atherosclerotic plaque formation, reflected by atherosclerotic inflammation, were increased in the ApoE(−/−) mice fed with a Western-type diet. APE1/Ref-1 expression was upregulated in aortic tissues of these mice, and was co-localized with cells positive for cluster of differentiation 31 (CD31) and galectin-3, suggesting endothelial cell/macrophage expression of APE1/Ref-1. Interestingly, APE1/Ref-1 plasma levels of ApoE(−/−) mice fed with a Western-type diet were significantly increased compared with those of the mice fed with normal diet (15.76 ± 3.19 ng/mL vs. 3.51 ± 0.50 ng/mL, p < 0.05), and were suppressed by atorvastatin administration. Correlation analysis showed high correlation between plasma APE1/Ref-1 levels and NLR, a marker of systemic inflammation. The cut-off value for APE1/Ref-1 for predicting atherosclerotic inflammation at 4.903 ng/mL showed sensitivity of 100% and specificity of 91%. We conclude that APE1/Ref-1 expression is upregulated in aortic endothelial cells/macrophages of atherosclerotic mice, and that plasma APE1/Ref-1 levels could predict atherosclerotic inflammation. MDPI 2020-09-21 /pmc/articles/PMC7555038/ /pubmed/32967121 http://dx.doi.org/10.3390/biomedicines8090366 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Yu Ran Joo, Hee Kyoung Lee, Eun-Ok Park, Myoung Soo Cho, Hyun Sil Kim, Sungmin Jin, Hao Jeong, Jin-Ok Kim, Cuk-Seong Jeon, Byeong Hwa Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title | Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title_full | Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title_fullStr | Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title_full_unstemmed | Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title_short | Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE(−/−) Mice |
title_sort | plasma ape1/ref-1 correlates with atherosclerotic inflammation in apoe(−/−) mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555038/ https://www.ncbi.nlm.nih.gov/pubmed/32967121 http://dx.doi.org/10.3390/biomedicines8090366 |
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