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Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells

Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This s...

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Autores principales: Sinha, Snehadri, Narjus-Sterba, Matilda, Tuomainen, Katja, Kaur, Sippy, Seppänen-Kaijansinkko, Riitta, Salo, Tuula, Mannerström, Bettina, Al-Samadi, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555061/
https://www.ncbi.nlm.nih.gov/pubmed/32899628
http://dx.doi.org/10.3390/ijms21186455
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author Sinha, Snehadri
Narjus-Sterba, Matilda
Tuomainen, Katja
Kaur, Sippy
Seppänen-Kaijansinkko, Riitta
Salo, Tuula
Mannerström, Bettina
Al-Samadi, Ahmed
author_facet Sinha, Snehadri
Narjus-Sterba, Matilda
Tuomainen, Katja
Kaur, Sippy
Seppänen-Kaijansinkko, Riitta
Salo, Tuula
Mannerström, Bettina
Al-Samadi, Ahmed
author_sort Sinha, Snehadri
collection PubMed
description Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression of ICAM1, ITGA3 or MMP1. On the other hand, HSC-3-conditioned media upregulated ICAM1 and MMP1 expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression.
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spelling pubmed-75550612020-10-14 Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells Sinha, Snehadri Narjus-Sterba, Matilda Tuomainen, Katja Kaur, Sippy Seppänen-Kaijansinkko, Riitta Salo, Tuula Mannerström, Bettina Al-Samadi, Ahmed Int J Mol Sci Article Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression of ICAM1, ITGA3 or MMP1. On the other hand, HSC-3-conditioned media upregulated ICAM1 and MMP1 expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression. MDPI 2020-09-04 /pmc/articles/PMC7555061/ /pubmed/32899628 http://dx.doi.org/10.3390/ijms21186455 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sinha, Snehadri
Narjus-Sterba, Matilda
Tuomainen, Katja
Kaur, Sippy
Seppänen-Kaijansinkko, Riitta
Salo, Tuula
Mannerström, Bettina
Al-Samadi, Ahmed
Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title_full Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title_fullStr Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title_full_unstemmed Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title_short Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
title_sort adipose-derived mesenchymal stem cells do not affect the invasion and migration potential of oral squamous carcinoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555061/
https://www.ncbi.nlm.nih.gov/pubmed/32899628
http://dx.doi.org/10.3390/ijms21186455
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