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Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests

Human strongyloidiasis is an important soil-transmitted helminthiasis that affects millions worldwide and can develop into fatal systemic strongyloidiasis in immunosuppressed patients. We have developed two new rapid and simple-to-use immunochromatographic test (ICT) kits for rapid serodiagnosis tha...

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Autores principales: Boonroumkaew, Patcharaporn, Sadaow, Lakkhana, Sanpool, Oranuch, Rodpai, Rutchanee, Thanchomnang, Tongjit, Phupiewkham, Weeraya, Intapan, Pewpan M., Maleewong, Wanchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555090/
https://www.ncbi.nlm.nih.gov/pubmed/32825495
http://dx.doi.org/10.3390/diagnostics10090615
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author Boonroumkaew, Patcharaporn
Sadaow, Lakkhana
Sanpool, Oranuch
Rodpai, Rutchanee
Thanchomnang, Tongjit
Phupiewkham, Weeraya
Intapan, Pewpan M.
Maleewong, Wanchai
author_facet Boonroumkaew, Patcharaporn
Sadaow, Lakkhana
Sanpool, Oranuch
Rodpai, Rutchanee
Thanchomnang, Tongjit
Phupiewkham, Weeraya
Intapan, Pewpan M.
Maleewong, Wanchai
author_sort Boonroumkaew, Patcharaporn
collection PubMed
description Human strongyloidiasis is an important soil-transmitted helminthiasis that affects millions worldwide and can develop into fatal systemic strongyloidiasis in immunosuppressed patients. We have developed two new rapid and simple-to-use immunochromatographic test (ICT) kits for rapid serodiagnosis that support stool examination for clinical diagnosis. Strongyloides stercoralis recombinant IgG immunoreactive antigen (GenBank: AAB97359.1; rSsIR-based ICT kit) was used for detection of IgG and IgG4 antibodies. The diagnostic efficacy of both kits was evaluated using human serum samples from strongyloidiasis patients, healthy individuals, and those with other parasitosis. At a prevalence of infection of 36.4%, the diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rSsIR-based IgG ICT kit were 91.7%, 83.8%, 76.4%, 94.6%, and 86.7%, respectively, and those of the rSsIR-based IgG4 ICT kit were 78.3%, 84.8%, 74.6%, 87.3%, and 82.4% respectively. The concordance between the two kits was 89.7%. The recombinant antigen can be produced to an unlimited extent and the kits can be used as point-of-care diagnostic tools and in large-scale surveys in endemic areas throughout tropical regions without necessitating additional facilities or ancillary supplies.
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spelling pubmed-75550902020-10-14 Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests Boonroumkaew, Patcharaporn Sadaow, Lakkhana Sanpool, Oranuch Rodpai, Rutchanee Thanchomnang, Tongjit Phupiewkham, Weeraya Intapan, Pewpan M. Maleewong, Wanchai Diagnostics (Basel) Article Human strongyloidiasis is an important soil-transmitted helminthiasis that affects millions worldwide and can develop into fatal systemic strongyloidiasis in immunosuppressed patients. We have developed two new rapid and simple-to-use immunochromatographic test (ICT) kits for rapid serodiagnosis that support stool examination for clinical diagnosis. Strongyloides stercoralis recombinant IgG immunoreactive antigen (GenBank: AAB97359.1; rSsIR-based ICT kit) was used for detection of IgG and IgG4 antibodies. The diagnostic efficacy of both kits was evaluated using human serum samples from strongyloidiasis patients, healthy individuals, and those with other parasitosis. At a prevalence of infection of 36.4%, the diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rSsIR-based IgG ICT kit were 91.7%, 83.8%, 76.4%, 94.6%, and 86.7%, respectively, and those of the rSsIR-based IgG4 ICT kit were 78.3%, 84.8%, 74.6%, 87.3%, and 82.4% respectively. The concordance between the two kits was 89.7%. The recombinant antigen can be produced to an unlimited extent and the kits can be used as point-of-care diagnostic tools and in large-scale surveys in endemic areas throughout tropical regions without necessitating additional facilities or ancillary supplies. MDPI 2020-08-20 /pmc/articles/PMC7555090/ /pubmed/32825495 http://dx.doi.org/10.3390/diagnostics10090615 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boonroumkaew, Patcharaporn
Sadaow, Lakkhana
Sanpool, Oranuch
Rodpai, Rutchanee
Thanchomnang, Tongjit
Phupiewkham, Weeraya
Intapan, Pewpan M.
Maleewong, Wanchai
Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title_full Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title_fullStr Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title_full_unstemmed Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title_short Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
title_sort effectiveness of strongyloides recombinant igg immunoreactive antigen in detecting igg and igg4 subclass antibodies for diagnosis of human strongyloidiasis using rapid immunochromatographic tests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555090/
https://www.ncbi.nlm.nih.gov/pubmed/32825495
http://dx.doi.org/10.3390/diagnostics10090615
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