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Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces

The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can ac...

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Autores principales: Bongaerts, Maud, Aizel, Koceila, Secret, Emilie, Jan, Audric, Nahar, Tasmin, Raudzus, Fabian, Neumann, Sebastian, Telling, Neil, Heumann, Rolf, Siaugue, Jean-Michel, Ménager, Christine, Fresnais, Jérôme, Villard, Catherine, El Haj, Alicia, Piehler, Jacob, Gates, Monte A., Coppey, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555211/
https://www.ncbi.nlm.nih.gov/pubmed/32911745
http://dx.doi.org/10.3390/ijms21186560
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author Bongaerts, Maud
Aizel, Koceila
Secret, Emilie
Jan, Audric
Nahar, Tasmin
Raudzus, Fabian
Neumann, Sebastian
Telling, Neil
Heumann, Rolf
Siaugue, Jean-Michel
Ménager, Christine
Fresnais, Jérôme
Villard, Catherine
El Haj, Alicia
Piehler, Jacob
Gates, Monte A.
Coppey, Mathieu
author_facet Bongaerts, Maud
Aizel, Koceila
Secret, Emilie
Jan, Audric
Nahar, Tasmin
Raudzus, Fabian
Neumann, Sebastian
Telling, Neil
Heumann, Rolf
Siaugue, Jean-Michel
Ménager, Christine
Fresnais, Jérôme
Villard, Catherine
El Haj, Alicia
Piehler, Jacob
Gates, Monte A.
Coppey, Mathieu
author_sort Bongaerts, Maud
collection PubMed
description The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery.
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spelling pubmed-75552112020-10-19 Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces Bongaerts, Maud Aizel, Koceila Secret, Emilie Jan, Audric Nahar, Tasmin Raudzus, Fabian Neumann, Sebastian Telling, Neil Heumann, Rolf Siaugue, Jean-Michel Ménager, Christine Fresnais, Jérôme Villard, Catherine El Haj, Alicia Piehler, Jacob Gates, Monte A. Coppey, Mathieu Int J Mol Sci Article The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery. MDPI 2020-09-08 /pmc/articles/PMC7555211/ /pubmed/32911745 http://dx.doi.org/10.3390/ijms21186560 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bongaerts, Maud
Aizel, Koceila
Secret, Emilie
Jan, Audric
Nahar, Tasmin
Raudzus, Fabian
Neumann, Sebastian
Telling, Neil
Heumann, Rolf
Siaugue, Jean-Michel
Ménager, Christine
Fresnais, Jérôme
Villard, Catherine
El Haj, Alicia
Piehler, Jacob
Gates, Monte A.
Coppey, Mathieu
Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title_full Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title_fullStr Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title_full_unstemmed Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title_short Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
title_sort parallelized manipulation of adherent living cells by magnetic nanoparticles-mediated forces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555211/
https://www.ncbi.nlm.nih.gov/pubmed/32911745
http://dx.doi.org/10.3390/ijms21186560
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