Cargando…
Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces
The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can ac...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555211/ https://www.ncbi.nlm.nih.gov/pubmed/32911745 http://dx.doi.org/10.3390/ijms21186560 |
_version_ | 1783593954790342656 |
---|---|
author | Bongaerts, Maud Aizel, Koceila Secret, Emilie Jan, Audric Nahar, Tasmin Raudzus, Fabian Neumann, Sebastian Telling, Neil Heumann, Rolf Siaugue, Jean-Michel Ménager, Christine Fresnais, Jérôme Villard, Catherine El Haj, Alicia Piehler, Jacob Gates, Monte A. Coppey, Mathieu |
author_facet | Bongaerts, Maud Aizel, Koceila Secret, Emilie Jan, Audric Nahar, Tasmin Raudzus, Fabian Neumann, Sebastian Telling, Neil Heumann, Rolf Siaugue, Jean-Michel Ménager, Christine Fresnais, Jérôme Villard, Catherine El Haj, Alicia Piehler, Jacob Gates, Monte A. Coppey, Mathieu |
author_sort | Bongaerts, Maud |
collection | PubMed |
description | The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery. |
format | Online Article Text |
id | pubmed-7555211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75552112020-10-19 Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces Bongaerts, Maud Aizel, Koceila Secret, Emilie Jan, Audric Nahar, Tasmin Raudzus, Fabian Neumann, Sebastian Telling, Neil Heumann, Rolf Siaugue, Jean-Michel Ménager, Christine Fresnais, Jérôme Villard, Catherine El Haj, Alicia Piehler, Jacob Gates, Monte A. Coppey, Mathieu Int J Mol Sci Article The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery. MDPI 2020-09-08 /pmc/articles/PMC7555211/ /pubmed/32911745 http://dx.doi.org/10.3390/ijms21186560 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bongaerts, Maud Aizel, Koceila Secret, Emilie Jan, Audric Nahar, Tasmin Raudzus, Fabian Neumann, Sebastian Telling, Neil Heumann, Rolf Siaugue, Jean-Michel Ménager, Christine Fresnais, Jérôme Villard, Catherine El Haj, Alicia Piehler, Jacob Gates, Monte A. Coppey, Mathieu Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title | Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title_full | Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title_fullStr | Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title_full_unstemmed | Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title_short | Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces |
title_sort | parallelized manipulation of adherent living cells by magnetic nanoparticles-mediated forces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555211/ https://www.ncbi.nlm.nih.gov/pubmed/32911745 http://dx.doi.org/10.3390/ijms21186560 |
work_keys_str_mv | AT bongaertsmaud parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT aizelkoceila parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT secretemilie parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT janaudric parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT nahartasmin parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT raudzusfabian parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT neumannsebastian parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT tellingneil parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT heumannrolf parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT siauguejeanmichel parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT menagerchristine parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT fresnaisjerome parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT villardcatherine parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT elhajalicia parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT piehlerjacob parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT gatesmontea parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces AT coppeymathieu parallelizedmanipulationofadherentlivingcellsbymagneticnanoparticlesmediatedforces |