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Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 differe...

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Autores principales: Rodríguez-López, Pedro, Filipello, Virginia, Di Ciccio, Pierluigi Aldo, Pitozzi, Alessandra, Ghidini, Sergio, Scali, Federico, Ianieri, Adriana, Zanardi, Emanuela, Losio, Marina Nadia, Simon, Ancuta Cezara, Alborali, Giovanni Loris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555242/
https://www.ncbi.nlm.nih.gov/pubmed/32825203
http://dx.doi.org/10.3390/foods9091141
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author Rodríguez-López, Pedro
Filipello, Virginia
Di Ciccio, Pierluigi Aldo
Pitozzi, Alessandra
Ghidini, Sergio
Scali, Federico
Ianieri, Adriana
Zanardi, Emanuela
Losio, Marina Nadia
Simon, Ancuta Cezara
Alborali, Giovanni Loris
author_facet Rodríguez-López, Pedro
Filipello, Virginia
Di Ciccio, Pierluigi Aldo
Pitozzi, Alessandra
Ghidini, Sergio
Scali, Federico
Ianieri, Adriana
Zanardi, Emanuela
Losio, Marina Nadia
Simon, Ancuta Cezara
Alborali, Giovanni Loris
author_sort Rodríguez-López, Pedro
collection PubMed
description The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 different heavy swine farms were surveyed, obtaining a total of n = 440 animal swabs and n = 150 environmental swabs. A total of n = 87 MRSA isolates were obtained, with an overall MRSA incidence of 17.50% (n = 77) among animal samples and a 6.67% (n = 10) among environmental. Molecular characterisation using multilocus sequence typing (MLST) plus spa-typing showed that sequence type ST398/t899 and ST398/t011 were the most commonly isolated genotypes, although other relevant sequence types such as ST1 or ST97 were also found. A lack of susceptibility to penicillins, tetracycline and ceftiofur was detected in >91.95, 85.05 and 48.28% of the isolates, respectively. Resistance to doxycycline (32.18%), enrofloxacin (27.59%) and gentamicin (25.29%) was also observed. Additionally, a remarkable level of antibiotic multiresistance (AMR) was observed representing a 77.01% (n = 67) of the obtained isolates. Genetic analysis revealed that 97.70% and 77.01% of the isolates harboured at least one antibiotic resistance or enterotoxin gene, respectively, pointing out a high isolate virulence potential. Lastly, 55.17% (n = 48) were able to produce measurable amounts of biofilm after 24 h. In spite of the current programmes for antibiotic reduction in intensively farming, a still on-going high level of AMR and virulence potential in MRSA was demonstrated, making this pathogen a serious risk in swine production chain, highlighting once more the need to develop efficient, pathogen-specific control strategies.
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spelling pubmed-75552422020-10-19 Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain Rodríguez-López, Pedro Filipello, Virginia Di Ciccio, Pierluigi Aldo Pitozzi, Alessandra Ghidini, Sergio Scali, Federico Ianieri, Adriana Zanardi, Emanuela Losio, Marina Nadia Simon, Ancuta Cezara Alborali, Giovanni Loris Foods Article The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 different heavy swine farms were surveyed, obtaining a total of n = 440 animal swabs and n = 150 environmental swabs. A total of n = 87 MRSA isolates were obtained, with an overall MRSA incidence of 17.50% (n = 77) among animal samples and a 6.67% (n = 10) among environmental. Molecular characterisation using multilocus sequence typing (MLST) plus spa-typing showed that sequence type ST398/t899 and ST398/t011 were the most commonly isolated genotypes, although other relevant sequence types such as ST1 or ST97 were also found. A lack of susceptibility to penicillins, tetracycline and ceftiofur was detected in >91.95, 85.05 and 48.28% of the isolates, respectively. Resistance to doxycycline (32.18%), enrofloxacin (27.59%) and gentamicin (25.29%) was also observed. Additionally, a remarkable level of antibiotic multiresistance (AMR) was observed representing a 77.01% (n = 67) of the obtained isolates. Genetic analysis revealed that 97.70% and 77.01% of the isolates harboured at least one antibiotic resistance or enterotoxin gene, respectively, pointing out a high isolate virulence potential. Lastly, 55.17% (n = 48) were able to produce measurable amounts of biofilm after 24 h. In spite of the current programmes for antibiotic reduction in intensively farming, a still on-going high level of AMR and virulence potential in MRSA was demonstrated, making this pathogen a serious risk in swine production chain, highlighting once more the need to develop efficient, pathogen-specific control strategies. MDPI 2020-08-19 /pmc/articles/PMC7555242/ /pubmed/32825203 http://dx.doi.org/10.3390/foods9091141 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-López, Pedro
Filipello, Virginia
Di Ciccio, Pierluigi Aldo
Pitozzi, Alessandra
Ghidini, Sergio
Scali, Federico
Ianieri, Adriana
Zanardi, Emanuela
Losio, Marina Nadia
Simon, Ancuta Cezara
Alborali, Giovanni Loris
Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title_full Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title_fullStr Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title_full_unstemmed Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title_short Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain
title_sort assessment of the antibiotic resistance profile, genetic heterogeneity and biofilm production of methicillin-resistant staphylococcus aureus (mrsa) isolated from the italian swine production chain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555242/
https://www.ncbi.nlm.nih.gov/pubmed/32825203
http://dx.doi.org/10.3390/foods9091141
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