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Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942

Carboxysomes are the specific CO(2)-fixing microcompartments in all cyanobacteria. Although it is known that the organization and subcellular localization of carboxysomes are dependent on external light conditions and are highly relevant to their functions, how carboxysome organization and function...

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Autores principales: Sun, Yaqi, Huang, Fang, Dykes, Gregory F., Liu, Lu-Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555275/
https://www.ncbi.nlm.nih.gov/pubmed/32872408
http://dx.doi.org/10.3390/life10090169
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author Sun, Yaqi
Huang, Fang
Dykes, Gregory F.
Liu, Lu-Ning
author_facet Sun, Yaqi
Huang, Fang
Dykes, Gregory F.
Liu, Lu-Ning
author_sort Sun, Yaqi
collection PubMed
description Carboxysomes are the specific CO(2)-fixing microcompartments in all cyanobacteria. Although it is known that the organization and subcellular localization of carboxysomes are dependent on external light conditions and are highly relevant to their functions, how carboxysome organization and function are actively orchestrated in natural diurnal cycles has remained elusive. Here, we explore the dynamic regulation of carboxysome positioning and carbon fixation in the model cyanobacterium Synechococcus elongatus PCC 7942 in response to diurnal light-dark cycles, using live-cell confocal imaging and Rubisco assays. We found that carboxysomes are prone to locate close to the central line along the short axis of the cell and exhibit a greater preference of polar distribution in the dark phase, coupled with a reduction in carbon fixation. Moreover, we show that deleting the gene encoding the circadian clock protein KaiA could lead to an increase in carboxysome numbers per cell and reduced portions of pole-located carboxysomes. Our study provides insight into the diurnal regulation of carbon fixation in cyanobacteria and the general cellular strategies of cyanobacteria living in natural habitat for environmental acclimation.
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spelling pubmed-75552752020-10-19 Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942 Sun, Yaqi Huang, Fang Dykes, Gregory F. Liu, Lu-Ning Life (Basel) Article Carboxysomes are the specific CO(2)-fixing microcompartments in all cyanobacteria. Although it is known that the organization and subcellular localization of carboxysomes are dependent on external light conditions and are highly relevant to their functions, how carboxysome organization and function are actively orchestrated in natural diurnal cycles has remained elusive. Here, we explore the dynamic regulation of carboxysome positioning and carbon fixation in the model cyanobacterium Synechococcus elongatus PCC 7942 in response to diurnal light-dark cycles, using live-cell confocal imaging and Rubisco assays. We found that carboxysomes are prone to locate close to the central line along the short axis of the cell and exhibit a greater preference of polar distribution in the dark phase, coupled with a reduction in carbon fixation. Moreover, we show that deleting the gene encoding the circadian clock protein KaiA could lead to an increase in carboxysome numbers per cell and reduced portions of pole-located carboxysomes. Our study provides insight into the diurnal regulation of carbon fixation in cyanobacteria and the general cellular strategies of cyanobacteria living in natural habitat for environmental acclimation. MDPI 2020-08-29 /pmc/articles/PMC7555275/ /pubmed/32872408 http://dx.doi.org/10.3390/life10090169 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Yaqi
Huang, Fang
Dykes, Gregory F.
Liu, Lu-Ning
Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title_full Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title_fullStr Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title_full_unstemmed Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title_short Diurnal Regulation of In Vivo Localization and CO(2)-Fixing Activity of Carboxysomes in Synechococcus elongatus PCC 7942
title_sort diurnal regulation of in vivo localization and co(2)-fixing activity of carboxysomes in synechococcus elongatus pcc 7942
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555275/
https://www.ncbi.nlm.nih.gov/pubmed/32872408
http://dx.doi.org/10.3390/life10090169
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