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Mechanisms for Curing Yeast Prions

Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded...

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Detalles Bibliográficos
Autores principales: Greene, Lois E., Saba, Farrin, Silberman, Rebecca E., Zhao, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555348/
https://www.ncbi.nlm.nih.gov/pubmed/32906758
http://dx.doi.org/10.3390/ijms21186536
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author Greene, Lois E.
Saba, Farrin
Silberman, Rebecca E.
Zhao, Xiaohong
author_facet Greene, Lois E.
Saba, Farrin
Silberman, Rebecca E.
Zhao, Xiaohong
author_sort Greene, Lois E.
collection PubMed
description Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded prion proteins are assembled into amyloid fibers or seeds, which are constantly severed and transmitted to daughter cells. To cure prions in yeast, it is necessary to eliminate all the prion seeds. Multiple mechanisms of curing have been found including inhibiting severing of the prion seeds, gradual dissolution of the prion seeds, asymmetric segregation of the prion seeds between mother and daughter cells during cell division, and degradation of the prion seeds. These mechanisms, achieved by using different protein quality control machinery, are not mutually exclusive; depending on conditions, multiple mechanisms may work simultaneously to achieve curing. This review discusses the various methods that have been used to differentiate between these mechanisms of curing.
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spelling pubmed-75553482020-10-19 Mechanisms for Curing Yeast Prions Greene, Lois E. Saba, Farrin Silberman, Rebecca E. Zhao, Xiaohong Int J Mol Sci Review Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded prion proteins are assembled into amyloid fibers or seeds, which are constantly severed and transmitted to daughter cells. To cure prions in yeast, it is necessary to eliminate all the prion seeds. Multiple mechanisms of curing have been found including inhibiting severing of the prion seeds, gradual dissolution of the prion seeds, asymmetric segregation of the prion seeds between mother and daughter cells during cell division, and degradation of the prion seeds. These mechanisms, achieved by using different protein quality control machinery, are not mutually exclusive; depending on conditions, multiple mechanisms may work simultaneously to achieve curing. This review discusses the various methods that have been used to differentiate between these mechanisms of curing. MDPI 2020-09-07 /pmc/articles/PMC7555348/ /pubmed/32906758 http://dx.doi.org/10.3390/ijms21186536 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Greene, Lois E.
Saba, Farrin
Silberman, Rebecca E.
Zhao, Xiaohong
Mechanisms for Curing Yeast Prions
title Mechanisms for Curing Yeast Prions
title_full Mechanisms for Curing Yeast Prions
title_fullStr Mechanisms for Curing Yeast Prions
title_full_unstemmed Mechanisms for Curing Yeast Prions
title_short Mechanisms for Curing Yeast Prions
title_sort mechanisms for curing yeast prions
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555348/
https://www.ncbi.nlm.nih.gov/pubmed/32906758
http://dx.doi.org/10.3390/ijms21186536
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