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The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555356/ https://www.ncbi.nlm.nih.gov/pubmed/33044554 http://dx.doi.org/10.1083/jcb.202004202 |
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author | Serra-Marques, Andrea Houtekamer, Ronja Hintzen, Dorine Canty, John T. Yildiz, Ahmet Dumont, Sophie |
author_facet | Serra-Marques, Andrea Houtekamer, Ronja Hintzen, Dorine Canty, John T. Yildiz, Ahmet Dumont, Sophie |
author_sort | Serra-Marques, Andrea |
collection | PubMed |
description | Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA’s C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell’s largest structures. |
format | Online Article Text |
id | pubmed-7555356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-75553562021-06-07 The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics Serra-Marques, Andrea Houtekamer, Ronja Hintzen, Dorine Canty, John T. Yildiz, Ahmet Dumont, Sophie J Cell Biol Article Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA’s C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell’s largest structures. Rockefeller University Press 2020-10-12 /pmc/articles/PMC7555356/ /pubmed/33044554 http://dx.doi.org/10.1083/jcb.202004202 Text en © 2020 Serra-Marques et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Serra-Marques, Andrea Houtekamer, Ronja Hintzen, Dorine Canty, John T. Yildiz, Ahmet Dumont, Sophie The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title | The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title_full | The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title_fullStr | The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title_full_unstemmed | The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title_short | The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics |
title_sort | mitotic protein numa plays a spindle-independent role in nuclear formation and mechanics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555356/ https://www.ncbi.nlm.nih.gov/pubmed/33044554 http://dx.doi.org/10.1083/jcb.202004202 |
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