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The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics

Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in...

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Autores principales: Serra-Marques, Andrea, Houtekamer, Ronja, Hintzen, Dorine, Canty, John T., Yildiz, Ahmet, Dumont, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555356/
https://www.ncbi.nlm.nih.gov/pubmed/33044554
http://dx.doi.org/10.1083/jcb.202004202
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author Serra-Marques, Andrea
Houtekamer, Ronja
Hintzen, Dorine
Canty, John T.
Yildiz, Ahmet
Dumont, Sophie
author_facet Serra-Marques, Andrea
Houtekamer, Ronja
Hintzen, Dorine
Canty, John T.
Yildiz, Ahmet
Dumont, Sophie
author_sort Serra-Marques, Andrea
collection PubMed
description Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA’s C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell’s largest structures.
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spelling pubmed-75553562021-06-07 The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics Serra-Marques, Andrea Houtekamer, Ronja Hintzen, Dorine Canty, John T. Yildiz, Ahmet Dumont, Sophie J Cell Biol Article Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA’s C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell’s largest structures. Rockefeller University Press 2020-10-12 /pmc/articles/PMC7555356/ /pubmed/33044554 http://dx.doi.org/10.1083/jcb.202004202 Text en © 2020 Serra-Marques et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Serra-Marques, Andrea
Houtekamer, Ronja
Hintzen, Dorine
Canty, John T.
Yildiz, Ahmet
Dumont, Sophie
The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title_full The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title_fullStr The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title_full_unstemmed The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title_short The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
title_sort mitotic protein numa plays a spindle-independent role in nuclear formation and mechanics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555356/
https://www.ncbi.nlm.nih.gov/pubmed/33044554
http://dx.doi.org/10.1083/jcb.202004202
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