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Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module

To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovere...

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Autores principales: Maksimov, Eugene G., Zamaraev, Alexey V., Parshina, Evgenia Yu., Slonimskiy, Yury B., Slastnikova, Tatiana A., Abdrakhmanov, Alibek A., Babaev, Pavel A., Efimova, Svetlana S., Ostroumova, Olga S., Stepanov, Alexey V., Slutskaya, Ekaterina A., Ryabova, Anastasia V., Friedrich, Thomas, Sluchanko, Nikolai N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555398/
https://www.ncbi.nlm.nih.gov/pubmed/32942578
http://dx.doi.org/10.3390/antiox9090869
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author Maksimov, Eugene G.
Zamaraev, Alexey V.
Parshina, Evgenia Yu.
Slonimskiy, Yury B.
Slastnikova, Tatiana A.
Abdrakhmanov, Alibek A.
Babaev, Pavel A.
Efimova, Svetlana S.
Ostroumova, Olga S.
Stepanov, Alexey V.
Slutskaya, Ekaterina A.
Ryabova, Anastasia V.
Friedrich, Thomas
Sluchanko, Nikolai N.
author_facet Maksimov, Eugene G.
Zamaraev, Alexey V.
Parshina, Evgenia Yu.
Slonimskiy, Yury B.
Slastnikova, Tatiana A.
Abdrakhmanov, Alibek A.
Babaev, Pavel A.
Efimova, Svetlana S.
Ostroumova, Olga S.
Stepanov, Alexey V.
Slutskaya, Ekaterina A.
Ryabova, Anastasia V.
Friedrich, Thomas
Sluchanko, Nikolai N.
author_sort Maksimov, Eugene G.
collection PubMed
description To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.
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spelling pubmed-75553982020-10-19 Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module Maksimov, Eugene G. Zamaraev, Alexey V. Parshina, Evgenia Yu. Slonimskiy, Yury B. Slastnikova, Tatiana A. Abdrakhmanov, Alibek A. Babaev, Pavel A. Efimova, Svetlana S. Ostroumova, Olga S. Stepanov, Alexey V. Slutskaya, Ekaterina A. Ryabova, Anastasia V. Friedrich, Thomas Sluchanko, Nikolai N. Antioxidants (Basel) Article To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery. MDPI 2020-09-15 /pmc/articles/PMC7555398/ /pubmed/32942578 http://dx.doi.org/10.3390/antiox9090869 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maksimov, Eugene G.
Zamaraev, Alexey V.
Parshina, Evgenia Yu.
Slonimskiy, Yury B.
Slastnikova, Tatiana A.
Abdrakhmanov, Alibek A.
Babaev, Pavel A.
Efimova, Svetlana S.
Ostroumova, Olga S.
Stepanov, Alexey V.
Slutskaya, Ekaterina A.
Ryabova, Anastasia V.
Friedrich, Thomas
Sluchanko, Nikolai N.
Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title_full Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title_fullStr Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title_full_unstemmed Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title_short Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
title_sort soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555398/
https://www.ncbi.nlm.nih.gov/pubmed/32942578
http://dx.doi.org/10.3390/antiox9090869
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