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Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current st...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555517/ https://www.ncbi.nlm.nih.gov/pubmed/32911690 http://dx.doi.org/10.3390/antiox9090838 |
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author | Fernandez-Robredo, Patricia González-Zamora, Jorge Recalde, Sergio Bilbao-Malavé, Valentina Bezunartea, Jaione Hernandez, Maria Garcia-Layana, Alfredo |
author_facet | Fernandez-Robredo, Patricia González-Zamora, Jorge Recalde, Sergio Bilbao-Malavé, Valentina Bezunartea, Jaione Hernandez, Maria Garcia-Layana, Alfredo |
author_sort | Fernandez-Robredo, Patricia |
collection | PubMed |
description | Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively counteracts the oxidative stress induced by hydrogen peroxide (H(2)O(2)). In addition, the increased levels of proinflammatory proteins such as Interleukin (IL)-6, IL-8, Monocyte chemoattractant protein (MCP)-1, Interferon (IFN)-γ, and tumor necrosis factor (TNF)-α triggered by lipopolysaccharide (LPS) exposure were significantly decreased by vitamin D addition. Interestingly, the increased IL-18 only decreased by vitamin D addition in endothelial cells but not in RPE cells, suggesting a main antiangiogenic role under inflammatory conditions. Moreover, H(2)O(2) and LPS induced the alteration and morphological damage of tight junctions in adult retinal pigment epithelium (ARPE-19) cells that were restored under oxidative and inflammatory conditions by the addition of vitamin D to the media. In conclusion, our data suggest that vitamin D could protect the retina by enhancing antioxidant defense and through exhibiting anti-inflammatory properties. |
format | Online Article Text |
id | pubmed-7555517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75555172020-10-19 Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells Fernandez-Robredo, Patricia González-Zamora, Jorge Recalde, Sergio Bilbao-Malavé, Valentina Bezunartea, Jaione Hernandez, Maria Garcia-Layana, Alfredo Antioxidants (Basel) Article Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively counteracts the oxidative stress induced by hydrogen peroxide (H(2)O(2)). In addition, the increased levels of proinflammatory proteins such as Interleukin (IL)-6, IL-8, Monocyte chemoattractant protein (MCP)-1, Interferon (IFN)-γ, and tumor necrosis factor (TNF)-α triggered by lipopolysaccharide (LPS) exposure were significantly decreased by vitamin D addition. Interestingly, the increased IL-18 only decreased by vitamin D addition in endothelial cells but not in RPE cells, suggesting a main antiangiogenic role under inflammatory conditions. Moreover, H(2)O(2) and LPS induced the alteration and morphological damage of tight junctions in adult retinal pigment epithelium (ARPE-19) cells that were restored under oxidative and inflammatory conditions by the addition of vitamin D to the media. In conclusion, our data suggest that vitamin D could protect the retina by enhancing antioxidant defense and through exhibiting anti-inflammatory properties. MDPI 2020-09-08 /pmc/articles/PMC7555517/ /pubmed/32911690 http://dx.doi.org/10.3390/antiox9090838 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fernandez-Robredo, Patricia González-Zamora, Jorge Recalde, Sergio Bilbao-Malavé, Valentina Bezunartea, Jaione Hernandez, Maria Garcia-Layana, Alfredo Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title | Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title_full | Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title_fullStr | Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title_full_unstemmed | Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title_short | Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells |
title_sort | vitamin d protects against oxidative stress and inflammation in human retinal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555517/ https://www.ncbi.nlm.nih.gov/pubmed/32911690 http://dx.doi.org/10.3390/antiox9090838 |
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