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Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells

Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current st...

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Autores principales: Fernandez-Robredo, Patricia, González-Zamora, Jorge, Recalde, Sergio, Bilbao-Malavé, Valentina, Bezunartea, Jaione, Hernandez, Maria, Garcia-Layana, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555517/
https://www.ncbi.nlm.nih.gov/pubmed/32911690
http://dx.doi.org/10.3390/antiox9090838
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author Fernandez-Robredo, Patricia
González-Zamora, Jorge
Recalde, Sergio
Bilbao-Malavé, Valentina
Bezunartea, Jaione
Hernandez, Maria
Garcia-Layana, Alfredo
author_facet Fernandez-Robredo, Patricia
González-Zamora, Jorge
Recalde, Sergio
Bilbao-Malavé, Valentina
Bezunartea, Jaione
Hernandez, Maria
Garcia-Layana, Alfredo
author_sort Fernandez-Robredo, Patricia
collection PubMed
description Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively counteracts the oxidative stress induced by hydrogen peroxide (H(2)O(2)). In addition, the increased levels of proinflammatory proteins such as Interleukin (IL)-6, IL-8, Monocyte chemoattractant protein (MCP)-1, Interferon (IFN)-γ, and tumor necrosis factor (TNF)-α triggered by lipopolysaccharide (LPS) exposure were significantly decreased by vitamin D addition. Interestingly, the increased IL-18 only decreased by vitamin D addition in endothelial cells but not in RPE cells, suggesting a main antiangiogenic role under inflammatory conditions. Moreover, H(2)O(2) and LPS induced the alteration and morphological damage of tight junctions in adult retinal pigment epithelium (ARPE-19) cells that were restored under oxidative and inflammatory conditions by the addition of vitamin D to the media. In conclusion, our data suggest that vitamin D could protect the retina by enhancing antioxidant defense and through exhibiting anti-inflammatory properties.
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spelling pubmed-75555172020-10-19 Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells Fernandez-Robredo, Patricia González-Zamora, Jorge Recalde, Sergio Bilbao-Malavé, Valentina Bezunartea, Jaione Hernandez, Maria Garcia-Layana, Alfredo Antioxidants (Basel) Article Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively counteracts the oxidative stress induced by hydrogen peroxide (H(2)O(2)). In addition, the increased levels of proinflammatory proteins such as Interleukin (IL)-6, IL-8, Monocyte chemoattractant protein (MCP)-1, Interferon (IFN)-γ, and tumor necrosis factor (TNF)-α triggered by lipopolysaccharide (LPS) exposure were significantly decreased by vitamin D addition. Interestingly, the increased IL-18 only decreased by vitamin D addition in endothelial cells but not in RPE cells, suggesting a main antiangiogenic role under inflammatory conditions. Moreover, H(2)O(2) and LPS induced the alteration and morphological damage of tight junctions in adult retinal pigment epithelium (ARPE-19) cells that were restored under oxidative and inflammatory conditions by the addition of vitamin D to the media. In conclusion, our data suggest that vitamin D could protect the retina by enhancing antioxidant defense and through exhibiting anti-inflammatory properties. MDPI 2020-09-08 /pmc/articles/PMC7555517/ /pubmed/32911690 http://dx.doi.org/10.3390/antiox9090838 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandez-Robredo, Patricia
González-Zamora, Jorge
Recalde, Sergio
Bilbao-Malavé, Valentina
Bezunartea, Jaione
Hernandez, Maria
Garcia-Layana, Alfredo
Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title_full Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title_fullStr Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title_full_unstemmed Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title_short Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells
title_sort vitamin d protects against oxidative stress and inflammation in human retinal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555517/
https://www.ncbi.nlm.nih.gov/pubmed/32911690
http://dx.doi.org/10.3390/antiox9090838
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