Definition of a Novel Plasmid-Based Gene Transfection Protocol of Mammalian Skeletal Muscles by Means of In Vivo Electroporation

We describe an original electroporation protocol for in vivo plasmid DNA transfection. The right hind limbs of C57 mice are exposed to a specifically designed train of permeabilizing electric pulses by transcutaneous application of tailored needle electrodes, immediately after the injection of pEGFP...

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Detalles Bibliográficos
Autores principales: Spugnini, Enrico P., Scimeca, Manuel, Amadio, Bruno, Cortese, Giancarlo, Fanciulli, Maurizio, Vincenzi, Bruno, De Luca, Antonio, Baldi, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555604/
https://www.ncbi.nlm.nih.gov/pubmed/32899477
http://dx.doi.org/10.3390/ijms21186494

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555604/
https://www.ncbi.nlm.nih.gov/pubmed/32899477
http://dx.doi.org/10.3390/ijms21186494

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