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Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to chara...

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Autores principales: Szilágyi, Melinda, Pös, Ondrej, Márton, Éva, Buglyó, Gergely, Soltész, Beáta, Keserű, Judit, Penyige, András, Szemes, Tomas, Nagy, Bálint
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555669/
https://www.ncbi.nlm.nih.gov/pubmed/32957662
http://dx.doi.org/10.3390/ijms21186827
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author Szilágyi, Melinda
Pös, Ondrej
Márton, Éva
Buglyó, Gergely
Soltész, Beáta
Keserű, Judit
Penyige, András
Szemes, Tomas
Nagy, Bálint
author_facet Szilágyi, Melinda
Pös, Ondrej
Márton, Éva
Buglyó, Gergely
Soltész, Beáta
Keserű, Judit
Penyige, András
Szemes, Tomas
Nagy, Bálint
author_sort Szilágyi, Melinda
collection PubMed
description Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field.
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spelling pubmed-75556692020-10-19 Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application Szilágyi, Melinda Pös, Ondrej Márton, Éva Buglyó, Gergely Soltész, Beáta Keserű, Judit Penyige, András Szemes, Tomas Nagy, Bálint Int J Mol Sci Review Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field. MDPI 2020-09-17 /pmc/articles/PMC7555669/ /pubmed/32957662 http://dx.doi.org/10.3390/ijms21186827 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Szilágyi, Melinda
Pös, Ondrej
Márton, Éva
Buglyó, Gergely
Soltész, Beáta
Keserű, Judit
Penyige, András
Szemes, Tomas
Nagy, Bálint
Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title_full Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title_fullStr Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title_full_unstemmed Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title_short Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
title_sort circulating cell-free nucleic acids: main characteristics and clinical application
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555669/
https://www.ncbi.nlm.nih.gov/pubmed/32957662
http://dx.doi.org/10.3390/ijms21186827
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