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Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to chara...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555669/ https://www.ncbi.nlm.nih.gov/pubmed/32957662 http://dx.doi.org/10.3390/ijms21186827 |
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author | Szilágyi, Melinda Pös, Ondrej Márton, Éva Buglyó, Gergely Soltész, Beáta Keserű, Judit Penyige, András Szemes, Tomas Nagy, Bálint |
author_facet | Szilágyi, Melinda Pös, Ondrej Márton, Éva Buglyó, Gergely Soltész, Beáta Keserű, Judit Penyige, András Szemes, Tomas Nagy, Bálint |
author_sort | Szilágyi, Melinda |
collection | PubMed |
description | Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field. |
format | Online Article Text |
id | pubmed-7555669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75556692020-10-19 Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application Szilágyi, Melinda Pös, Ondrej Márton, Éva Buglyó, Gergely Soltész, Beáta Keserű, Judit Penyige, András Szemes, Tomas Nagy, Bálint Int J Mol Sci Review Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field. MDPI 2020-09-17 /pmc/articles/PMC7555669/ /pubmed/32957662 http://dx.doi.org/10.3390/ijms21186827 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Szilágyi, Melinda Pös, Ondrej Márton, Éva Buglyó, Gergely Soltész, Beáta Keserű, Judit Penyige, András Szemes, Tomas Nagy, Bálint Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title | Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title_full | Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title_fullStr | Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title_full_unstemmed | Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title_short | Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application |
title_sort | circulating cell-free nucleic acids: main characteristics and clinical application |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555669/ https://www.ncbi.nlm.nih.gov/pubmed/32957662 http://dx.doi.org/10.3390/ijms21186827 |
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